Zeeshan Zaman | Freelancer Writing Work

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BIO INFORMATICS GROUP MEMBERS: ARSHI QAYYUM MEHREEN ZAMAN HAREEM MIHAD MAIMOONA SAIF LALEENA JAN ACTIVITY 1# ENSEMBLE ACTIVITY 2# CLUSTAL W ACTIVITY 3# BLAST ACTIVITY 4# PRMER 3 WEB ACTIVITY 5# PRIMARY STRUCTURE PREDICTION ACTIVITY 6# SECONDARY STRUCTURE PREDICTION ACTIVITY 7# TERTIARY STRUCTURE PREDICTION ACTIVITY 8# MUTATION ACTIVITY#1 Gene: BRCA1 ENSG- Description BRCA1 DNA repair associated [Source:HGNC Symbol;Acc:HGNC:1100] Gene Synonyms BRCC1, FANCS, PPP1R53, RNF53 Location Chromosome 17: 43,044,295-43,170,245reverse strand. GRCh38:CM- About this gene This gene has 41 transcripts (splice variants), 198 orthologues and is associated with 148 phenotypes. Transcript Table Transcript ID Name bp Protein Biotype CCDS UniProt Match RefSeq Match Flags ENST- BRCA- No protein Protein coding CDS not defined - - - ENST- BRCA- No protein Protein coding CDS not defined - - - ENST- BRCA- No protein Retained intron - - - ENST- BRCA-aa Nonsense mediated decay - - - ENST- BRCA-aa Protein coding - - GENCODE basic ENST- BRCA- No protein Retained intron - - - ENST- BRCA- No protein Retained intron - - - ENST- BRCA- No protein Retained intron - - - ENST- BRCA-aa Protein coding A0A494C182 - CDS 3' incomplete ENST- BRCA-aa Protein coding A0A2R8Y587 - CDS 3' incomplete ENST- BRCA-aa Protein coding A0A2R8Y7V5 - CDS 5' incomplete ENST- BRCA-aa Nonsense mediated decay A0A2R8Y6Y9 - - ENST- BRCA-aa Protein coding A0A0U1RRA9 - TSL:5CDS 3' incomplete ENST- BRCA- No protein Protein coding CDS not defined - - TSL:5 ENST- BRCA-aa Protein coding G1UI37 - TSL:1CDS 3' incomplete ENST- BRCA-aa Protein coding K7EJW3 - GENCODE basicTSL:1 ENST- BRCA-aa Protein coding K7EPC7 - GENCODE basicTSL:1 ENST- BRCA-aa Protein coding C6YB45 - GENCODE basicTSL:1 ENST- BRCA-aa Protein coding C9IZW4 - TSL:1CDS 3' incomplete ENST- BRCA-aa Protein coding Q3B891 - TSL:1CDS 3' incomplete ENST- BRCA-aa Protein coding B7ZA85 - TSL:2CDS 3' incomplete ENST- BRCA-aa Protein coding CCDS11459 P38398-8 - GENCODE basicTSL:5 ENST- BRCA-aa Nonsense mediated decay E9PC22 - TSL:1 ENST- BRCA-aa Protein coding CCDS11454 P38398-3 - GENCODE basicTSL:5 ENST- BRCA-aa Protein coding E7ENB7 - TSL:4CDS 3' incomplete ENST- BRCA-aa Protein coding H0Y8B8 - GENCODE basicTSL:5 ENST- BRCA-aa Protein coding E7EUM2 - TSL:1CDS 3' incomplete ENST- BRCA-aa Protein coding E9PH68 - TSL:1CDS 3' incomplete ENST- BRCA-aa Protein coding E7EQW4 - TSL:5CDS 3' incomplete ENST- BRCA-aa Protein coding H0Y8D8 - TSL:2CDS 5' and 3' incomplete ENST- BRCA- No protein Retained intron - - TSL:3 ENST- BRCA-aa Protein coding CCDS11456 P38398-7 - GENCODE basicAPPRIS ALT2TSL:1 ENST- BRCA-aa Protein coding E7EWN5 - TSL:1CDS 3' incomplete ENST- BRCA-aa Protein coding CCDS11455 P38398-6 - GENCODE basicTSL:1 ENST- BRCA-aa Nonsense mediated decay P38398-2 - TSL:3 ENST- BRCA-aa Protein coding H0Y850 - TSL:2CDS 5' and 3' incomplete ENST- BRCA-aa Nonsense mediated decay P38398-2 - TSL:1 ENST- BRCA-aa Non stop decay A0A0A0MSN1 - TSL:1CDS 5' incomplete ENST- BRCA-aa Protein coding CCDS11453 P38398 NM_- MANE SelectEnsembl CanonicalGENCODE basicAPPRIS P2TSL:1 ENST- BRCA-aa Protein coding Q5YLB2 - GENCODE basicTSL:1 ENST- BRCA-aa Protein coding P38398-5 - GENCODE basicTSL:5 Gene Tree Human Sequence >sp|P38398-5|BRCA1_HUMAN Isoform 5 of Breast cancer type 1 susceptibility protein OS=Homo sapiens OX=9606 GN=BRCA1 MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQ CPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKD EVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLGTVRTLRTKQRIQPQKTSVYI ELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKGEAASGCESETSVSEDC SGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDL RNPEQSTSEKAVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCP SLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTP YLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQSPAAAHTTDT AGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITE ETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVN GRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGT GVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSH Y Orthologus Black snub-nosed monkey >tr|A0A2K6MZL2|A0A2K6MZL2_RHIBE Breast cancer type 1 susceptibility protein homolog OS=Rhinopithecus bieti OX=61621 GN=BRCA1 PE=4 SV=1 MDLSAVRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCRFCMLKLLNQKKGPSQ CPLCKNDITKRSLQESTRFSQLVEELLKIIHAFQLDTGLQCNAVIMGMRYHSQPQRFYTE PANNCLTQETSLSVPLSNFGIVRTLRTKQRIQPQKKSVYIELGSDSSEDMVNKATYCSVG DQELLQITPQGTRDETSLDSAKKAACEFSEKDITNTEHHQSSNNDLNTTEKHATERHPEK YQGSSVSNLHVEPCGTNTHASSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARNQHNR WTGSKETCNDRQTPSTEKKVDLNADTLYERKERNKQKLPCSENPRDTEDVPWITLNSSIQ KVNEWFSRSDELLSSDDSHDGGSESNAKVADVLDVLNEVDEYSGSSEKIDLLASDPHEPL ICKSERVHSSSVESNIEDKIFGKTYRRKANLPNLSHVTENLIIGALVTESQIMQERPLTN KLKRKRRTTSGLHPEDFIKKADLAVQKTPEMINQGTNQMEQNGQVMNITNSAHENKTKGD SIQNEKNPDPIESLEEESAFKTKAEPISSSINNMELELNIHNSKAPKKNRLRRKSSTRHI HALELVVSRNLSPPNCTELQIDSCSSSEEIKKKNYNQMPVRHSRKLQLMEDKESATGAKK SNKPNEQTSKRHASDTFPELKLTKVPPSFTNCSNTSELKEFVNPSLSREEKEEKLETVKV SNNAKDPKDLMLSGERVLQTERSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEQNK CVSQCAAFENPKELIHGCSEDTRNDTEGFKYPLGREVNHSQETSIEIEESELDTQYLQNT FKVSKRQSFALFSNPGNPEEECATFSAHSKSLKKQSPKVTSECEQKEENQGKKESNIKPV QTVNITAGFPVVCQKDKPVDNAKCSIKGGSRFCLSSQFRGNETGLITPNKHGLLQNPYHI PPLFPVRSFVKTKCKKNLLEENSEEHSVSPERTVGNENIIPSTVSTISHNNIRENAFKEA SSSNINEVGSSTNEVGSSINEVGSSDENIQAELGRNRGPQLNAVLRLGLLQPEVCKQSLP ISNCKHPEIKKQEHEEVVQTVNTDFSPCLISDNLEQPMGSSHVSEVCSETPDDLLDDGEI KEDTSFAENDIKESSAVFSKSVQRGELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSE DEELPCFQHLLFGKVSNIPSQTTRHSTVATECLYKNTEENLLSLKNSLTDCSNQVILAKA SQEHHLSEETKGSGSLFSSQCSELEDLTANTNTQDPFLIGSSKRMRHQSESQGVGLSDKE LVSDDEERGTGLEEDNPEEQSVDSDLGEAASGYESETSVSEDCSRLSSQSEILTTQQRDT MQDNLIKLQQEMAVLEAVLEQHGSQPSNSYPSIVTDSSALEDLRNPEQSTSEKGICFLIP FKAVLTSQKSSEYPISQNPEGFSADKFKVSADSSTSKNKEPGVERSSPSKCQSLEDRWYV HSSSGSLQNGNYPSQEELIKVVDMEEQQLEKSGPHNLMEPSYLPRQDLEGTPYLESGISL FSDDPESDPSEDRALESAHVGSIPSSTSALKVPQWQVAESAQSPAAAHNTNTAEYNAMEE SVSREKPKLTASTERVNKRMSLVVSGLTPEEFMLVYKFARRYHIALTNLITEETTHIVMK TGIPRTFTEYFAFAHDFEVRGDVVNGRNHQGPKRARESPDRKIFRGLEICCYGPFTNMPT DQLEWMVQLCGASVVKELSSFTLGTVSICCPVREEGHTIGQMCEAPVVTREWVLDSVALY QCQELDAYLIPQIPHSHY Mouse >sp|P48754|BRCA1_MOUSE Breast cancer type 1 susceptibility protein homolog OS=Mus musculus OX=10090 GN=Brca1 PE=1 SV=3 MDLSAVQIQEVQNVLHAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQ CPLCKNEITKRSLQGSTRFSQLAEELLRIMAAFELDTGMQLTNGFSFSKKRNNSCERLNE EASIIQSVGYRNRVRRLPQVEPGNATLKDSLGVQLSNLGIVRSVKKNRQTQPRKKSVYIE LDSDSSEETVTKPGDCSVRDQELLQTAPQEAGDEGKLHSAEEAACEFSEGIRNIEHHQCS DDLNPTENHATERHPEKCQSISISNVCVEPCGTDAHASSLQPETSSLLLIEDRMNAEKAE FCNKSKQPGIAVSQQSRWAASKGTCNDRQVPSTGEKVGPNADSLSDREKWTHPQSLCPEN SGATTDVPWITLNSSVQKVNEWFSRTGEMLTSDSASARRHESNAEAAVVLEVSNEVDGGF SSSRKTDLVTPDPHHTLMCKSGRDFSKPVEDNISDKIFGKSYQRKGSRPHLNHVTEIIGT FITEPQITQEQPFTNKLKRKRSTSLQPEDFIKKADSAGVQRTPDNINQGTDLMEPNEQAV STTSNCQENKIAGSNLQKEKSAHPTESLRKEPASTAGAKSISNSVSDLEVELNVHSSKAP KKNRLRRKSSIRCALPLEPISRNPSPPTCAELQIDSCGSSEETKKNHSNQQPAGHLREPQ LIEDTEPAADAKKNEPNEHIRKRRASDAFPEEKLMNKAGLLTSCSSPRKSQGPVNPSPQR TGTEQLETRQMSDSAKELGDRVLGGEPSGKTTDRSEESTSVSLVSDTDYDTQNSVSVLDA HTVRYARTGSAQCMTQFVASENPKELVHGSNNAGSGTEGLKPPLRHALNLSQEKVEMEDS ELDTQYLQNTFQVSKRQSFALFSKPRSPQKDCAHSVPSKELSPKVTAKGKQKERQGQEEF EISHVQAVAATVGLPVPCQEGKLAADTMCDRGCRLCPSSHYRSGENGLSATGKSGISQNS HFKQSVSPIRSSIKTDNRKPLTEGRFERHTSSTEMAVGNENILQSTVHTVSLNNRGNACQ EAGSGSIHEVCSTGDSFPGQLGRNRGPKVNTVPPLDSMQPGVCQQSVPVSDKYLEIKKQE GEAVCADFSPCLFSDHLEQSMSGKVFQVCSETPDDLLDDVEIQGHTSFGEGDIMERSAVF NGSILRRESSRSPSPVTHASKSQSLHRASRKLESSEESDSTEDEDLPCFQHLLSRISNTP ELTRCSSAVTQRMPEKAEGTQAPWKGSSSDCNNEVIMIEASQEHQFSEDPRCSGSMFSSQ HSAAQGSTANANSQDSNFIPPSKQRSHQCGNEEAFLSDKELISDNEEMATCLEEDNDQEE DSIIPDSEASGYESETNLSEDCSQSDILTTQQRATMKYNLIKLQQEMAHLEAVLEQRGNQ PSGHSPSLLADPCALEDLPDLEPNMSGAAILTSKNINENPVSQNLKSACDDKFQLQHLEG PTSGDDESGMGRPSPFKSPLAGSRGSAHGCSRHLQKRNSPSQEELLQPAGSEASSEPHNS TGQSCLPRRELEGTPYLGSGISLFSSRDPESESPKEPAHIGTTPASTSALKIPQGQVAFR SAAAAGADKAVVGIVSKIKPELTSSEERADRDISMVVSGLTPKEVMTVQKFAEKYRLTLT DAITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVVRSIQERRLLNVHEFEVK GDVVTGRNHQGPRRSRESREKLFKGLQVYCCEPFTNMPKDELERMLQLCGASVVKELPSL THDTGAHLVVIVQPSAWTEDSNCPDIGQLCKARLVMWDWVLDSLSSYRCRDLDAYLVQNI TCDSSEPQDSND Cat >tr|M3WIB0|M3WIB0_FELCA Breast cancer type 1 susceptibility protein homolog OS=Felis catus OX=9685 GN=BRCA1 PE=4 SV=4 MDLSADRVEEVQSVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQRKGPSQ CPLCKNDITKRSLQESTRFSQLVEELLNIIHAFELDTGLQFANSYNFSKKENNSPEHLKE EVSIIQSMGYRNRAKRLRQSEPENPNLETSLSVQLSNFGIVRSLRTKQQIQPQNKSVYIE LGSDSSEDTVNKASYFSAEDDELLGITPQGARAEASLNPAKKASCEFSEDITNIEHRQPS NKDLTATEKHATEKHPEKYQGISVSNLHVEPCATNTRASSLQHENSSLLLTKGRMNVEKA EFCNESKQPGVARSQQSRWAESKETCNDRQTPSTEKKVVLNADPLCRRKLSKQKSPCSDS PRDSQDVPWITLNSSIQKVNEWFSRSDEMLTSDDSHDEGSESNPGVAGAEVPNVVDGYSG SSEKIDLMASDPHDALICESERVHTKPVESNIEDKIFGKTYRRKASLPNLSHTSEDLIIG ACAIEPQITQAYPLTNKTKRKRRSTAGLHPEDFIKKVDLAIVQKTPEKLIEGTDRIGQNG HVMNRTNNGPENGTKGDYVQKEKNAKPTESLEKESAFRTKAEPISSNISNMESELNSHSS RAPKENRLRRKSSTRHMRALELVVNRNPSPPDHTELQIDSCSSSEEMVKRKSSEQMLVRH SKTLQLVENKEPAPGAKKRNKPSKQINKRLASNTFPELNLTNIPGVFTNCSSSNTLQEFV NPGIQREELEESRGTIHVSDRTRDPKALVSSGGRSLQTERSVESTSISLVPDADYGTQDS ISLLEADTLGKAKTAPNQRVSLCAAIENPKEAIRGCSKDTRNGTESFTDPLRREDNHTQE TSIEMEENELDTQCLYNTFKVSKRQSFALFSNPGNPEKECATAWARSTSLRKQSPKVALE YEQKEENQGKKQSEIKHVQAVRATAGFSAVSQKVEKPGDYAKCSIKGVPGLCQSSQFRGN ETELFIANNHDISKNPYHIPPLSPIRSSVNAVCKKNLSEEKFEQRSMSPERAVGNESVIQ STVNTISQNNIRENTFKEVSSSSVNEVGSSANEVGSSINEVGSSGENIQTELGRNRGPKL SAMLRLGLMQPEVYEQSLPISNCKCPEMKRQGENEGVVQSVNADFSPCLISDNVEQPMES SRASQVCSETPNDLLNGDEIKGKISFAESAKERSAVFGKSVQKGEFRRSPSPLDHTHLAQ GHQTETRKLESSEENVSSEEEELPCFQHLLFGKVTSIPSQSTSHNAIATEGLSKKTEANL ESLKDSLHDCSNQVTSAKESQEHHLSEEARCSGSLFSSQCSASEDSAVNTNTQDPFLMFD PPSKQVRHQSENVDVLSDEELVSYDDEREPGLEEDNHEEEQSVDSNLGEAASGYENETSL SEDCSGVSSQSDILTTQQRDTMQDNLIKLQQEMAELEAVLEQHESQPSNSSPALIRDSYA PEDLFNLEQNTSEKAVLTSEKSSDCLISQNPESLSADKFQVSLDSSTSKTKEPGMRRSSP SQSRLLDTRQCVRSHSSSLQSTNCLSQKELIKAVDGEELQLTKSEPRDFMEQSYLPRQDL EGTPYFESGISLFSDDPESDFSVDRAPEPVHVSSMPTSTSALKLPQFQVEESAKSTAAVH ITNTAGYNKREDIEGKERPEMMSSTKRVNKRISMVASGLTPKELVLVQKFARRHQITLTN VISEETTHVIMKTDAEFVCERTLKYFLGIAGGRWVVSYFWVTQSIKERKMLDEHDFEVRG DVVNGRNHQGPKRARESRDRKIFGGLEICCYGPFTNMPTDQLEWMVRLCGASVVKRPSLF TLSKATHPVVVVQPDAWTEDSGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQI PRATADLSQPCV Elephant >tr|G3TDF5|G3TDF5_LOXAF Breast cancer type 1 susceptibility protein homolog OS=Loxodonta africana OX=9785 GN=BRCA1 PE=4 SV=1 MDFSVAHIEEVQNVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQ CPLCKNHITKRSLQESTRFSQLVEELLKIIRAFELDTGLQFANSYNFAKKENNSPEHVKE EVSIIQSMGYRNRAKRLRQSEPENPTLQETSLNAQLSNFGIVRSLRTKQHTQLQNKSVYI ELGSDSSEDTVNKESYYSVVDQELLHIAPQGAKAEASLDPTKKAACEFSEKDITNLEYRQ SSNKDLNTIKKHAAEKHPEKYRGISVSNLNVEPCGTNTHASSLQHKNSSLLLTKDRMNVE KAEFCNKSKQPGLARSQQSRWAESKETCNDRQTPSTEKKVDVNADPLYERKEVNKQKPPR SENLRDTQDIPWITLNSSIQKVNEWFFRSDGLDVLNDEGPESSAEVAGALEVPNEVHSNS SEKIDLMASDLHGALICESERVPSKPAESNIEDKIFGKTYRRKAGMSHLSHITEDLIMGA IASEPQIAREHPFTNKLKRKRRTSGLHPEDFIKKVDLAVVQKIPEKINQETDHVEQNGQV MNIANGGRENETKGDYVQKEKNAIPTESLAKESAFRTKAEPISSSISNMELELNMHNSKA PKKNRLRRKSSTRHIHALELVVNRNPSPPTHTELQIDSWSSSEETKKKSSEQKPIRHNRN LQLMKNQETATGAKKSNKPKEQISKRHGADSYPELHLTTTAGFITKCSSSDNLQEFVNPS LQGEKTEENLETIQVSNITKEPKDLVLNGGRDLQTKKSIESTNISVIPDTVYGTQDSVSL LGADTPGKAKTAPNRCASQCTAIENPSELTNSCPKDTRNDTEGFKDLLRCEASHIQETCI EIEESELDTQYLQSTFKVSKRQSFALFSNPEKKCATICAHSKSLRKQSPKVTLVCGEKEE NQGNREPKIKHEQAVHMPTGYPEACQKEKPSDYTKYSIKGVSGLCQSSQFRGSESELITA DGHGISQNPDQIPSLSPTRSSVKTKCKTNLLEERFEEHTISLERAMRNENVIQSTVSTVS QNNIRESASKEASSSSINEVCSSINEVGSSGENIQAEISRKRGPKLNAVLRLGLMQPEVY KQSLPISDCKHPGIKTQGENEGVVQAVNTDFSPCLISDNLEQPVGTSRASQVCSETPDDL LDDDEIKENISFAENGIKERSVFIKDDQRREFRRNPSPLSHSGLAQGCLRGARELEPSQE NISSEDEELPCFQHLLFGEVTNIPSQSTRHNADAIQCLSKNTEENLGSVQNSINDCSNQV TSAKASQERHLSEEVRCSSSLFSSQCSVMEDLATNTNTQDPFLMFGSPSKQARHQSENQE VVLSDEELVSDEYKRGPGLEEDNHQDDQSVDSNLDEVASGYESESSLSEAGSGPSSQSDI LTTQQRDTMQDNLIKLQQEMAELEAVLEQQGSQPSNSSPSLLAGSRAPKNLLNPEQNTSE KGSISTSEKSNEHPVSENPEGLSADKFRPSLDSATSKNKDPGMERSSPSKFQLLDNNRWD VHSHSRSLQNGNRLSQEELVKIVDVEEQQVEKSEARGLIEQPYLPLQDLEGTPCLESGVS LFSDDPESDPSEDRAPDPAHVCSSPASSSALKLPQFQVAESASDSADAHTNNTTAYNARE ESVNKETPGVTSSAERANRRASMVASGLTQKEFMLVHKFARKHHCTLTNQITEETTHVIM KTDADFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLDECDFEVRGDVVNGRNHQGP KRAREFQDRKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPLSFTLGTGTHPIVV VQPDAWTEDSGFHAIGQLCEAPVVTREWVLDSVALYQRQELDTYLIPQIPHSHY Duck >tr|A0A493TLC3|A0A493TLC3_ANAPP RING-type E3 ubiquitin transferase OS=Anas platyrhynchos platyrhynchos OX=8840 GN=BRCA1 PE=4 SV=1 MDLAVVAAGEVQNVLSAMQKNLECPICLDVVKEPVSTKCDHIFCRFCMYKLLSKKKKGVV QCPLCKTEVTKRSLTENSRFKQLIEGLLEAIHAFELDTGVKFLNSHRLPKSSTEATAAKL PCNESSVIQSKGFRNRKRSAKENEQENCSLQGANVDAQRADISVKRCSLRNRNQKHDLEK GVFIELGTDSSEELCKQAGNTGLGNKEVVQISSEEKLEEPKSPEKGNEYSCNTRPTELRA KEIILPNVIGESDFSKEALSKKSMQSVAEHANPDQVNEMECQSSPLNILSPDLLTERCDR ANNASPLKDGDTSLSKNAEETGAEQTQCSNKSQEFDLEDSSENRPGKSKEIDAVVQSVED VEMCESENNSEKESPLEKLLQPETPHCPTLHQVSRKRLKRSIQKVNEWFSKSDEILSSNS SHDDPAEASDVSGEGDALSDKDSCISEKTSPVVGFMETTVSEGNKRWSKEKTDNIKDKIF GKTYERERKSNPPIIMREILPTTKKEDVPAVQECLNNSRKDSLKRKRKPVRHLQPEDFIK KKDVEEAGGCPQSVNTCLGDAEKKKCEESSALKENPPPEKRRGSTLAEFEEGASMSKNAT EKVTCEHSGELELNNSDQKSTKNPSSAAKRCRRSTRTMCALQLVVDKNSASPDPAETLIE SYPSSEEPRNADSEQRQVRRSRRLQLLSEEMTKETAKRAVIKEASKYDGDQEGPVFGVEK TVLVRDSECKDLCEQQDISSCKPLTNLESADLEANAIQMSPKNSSETAKTGKRLFNPAPS HQHSDLNSSSPRAGSEEGEVLGDPFLHQSPSKTVVQTASHLPEGKRAESCPTLPQDKEHS TQNVPEGLRTEKSPMAKNVSELAKESEDSEQDTQYLRNIFRNSKRLSFSLYPAPRKACEI DDVASEKLKMSYADQVEKRHSKYFNTENLQKEKTAENLSNVCEKEKMKNCESACASPVSC TVSNAECACSGDCQDVSQVANQGNLMTPVRMGAASSEGKNRPQKGERGSEKTASTDAGAE SVLRQNPVKSSKSQSVQSNTEEHVFQRTDLNTVSETCFSSESDQAEKAEFVDSEGPVLCF QPGSMARPTACQQRSAEFNCKDAEKRRSKRRRLIGDEEPATQTASTGVPECLVAETLEET FKGGCSDTSETPDGLMCSDDDSEENVSFCEAPRKDSSAVFVKSDLVKERDHGGVGSQPRS TGIQRARRRAQKLQSSSEESSEDEDLPCFQALIFGKSASTPLQTNKQVTSALGSSGNPNT LPRGGSREDKVQKTPEAALSNGCASPSQESECSVNLFSSQSNVSEESADGAQEPKKPLLQ VHASKQQSNVSESKETLQSCAGRLKRRKTNSKDECQEDPNLGEASGYDSETSHVEDSCGP FSQGEILSTQQKNAMQNNLKKLQQEMAALEAVLQEHGSQDSEPLPACRELLGPRSERTLA VEQTRQRGAESTSEGSLENHKCSTSKGFSADEVHVIPSDSSNSEVKELEIERSPELPYPS LKSSWSKRPDLEEDLRHDSVVENKAPQETKPVHEAVQEYSQSEEENADEKSGTKLNKASV LNDLCGNVTRSPHASSSFVKPPHLQTAEATNSSAVVQNDNKSSSQGCKSKRSVCFPTSVL HNAYGKENATNPVVTNRKKMSIVASGLKQSEHMVVQKFARKTQSTFSNHITDGTTHVIMK TDKELVCERTLKYFLGIAGRKWVVSYQWVVQSFKEGMILDEENFEVRGDVINGRNHQGPK RARQSLTEKIFKDFEICCYGPFTDMTTGHLEWMVELCGASVVKQLHLFTHKMNSTAVVVV QPDAWMEGTSYKAIQQKNNVAVVTREWVLDSVACYECQELDAYLVS ACTIVITY#2 CLUSTAL 2.1 multiple sequence alignment Human SPP-BRCAHUMANISOFORMOFB-REASTCANCER-TYPESU-----SCEPTIBILITYP Cat ---TRMWIB--MWIB--FELCAB-REASTCANCER-TYPESU-----SCEPTIBILITYP Elephant -------TRGTDFGTDFLOXAFB-REASTCANCER-TYPESU-----SCEPTIBILITYP Black ----TRAAKMZLAAKMZLRHIBEBREASTCANCER-TYPESU-----SCEPTIBILITYP Mouse -----------SPPBRCAMOUSEBREASTCANCER-TYPESU-----SCEPTIBILITYP Duck --------------TRAATLCAATLCANAPPRING-TYPEEUBIQUITINTRANSFERAS *.: .. : ****.* : :. : . Human ROTEINO------------SHOMOSAPIENSOXG-NB-----RCAMDLSALRVEEVQNVI Cat ROTEINHOMOLOG-----OSFELISCATUSO--X-GNBRCAPESVMDLSADRVEEVQSVL Elephant ROTEINHOMOLOGOSLOXODONTAAFRICANAOX-GNBRCAPESVMDFSVAHIEEVQNVL Black ROTEINHOMOLOGOSRHINOPITHECUSBIETIOXGNBRCAPESVMDLSAVRVEEVQNVI Mouse ROTEINHOMOLOGO-------SMUSMUSCULUSOXGNBRCAPESVMDLSAVQIQEVQNVL Duck EOSANASPLATYRHYNCHOSPLATYRHYNCHOSOXGNBRCAPESVMDLAVVAAGEVQNVL .*: . ...**::. ***.*: Human NAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKK-GPSQCPLCKNDITKRSLQ Cat NAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQRK-GPSQCPLCKNDITKRSLQ Elephant NAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKK-GPSQCPLCKNHITKRSLQ Black NAMQKILECPICLELIKEPVSTKCDHIFCRFCMLKLLNQKK-GPSQCPLCKNDITKRSLQ Mouse HAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKK-GPSQCPLCKNEITKRSLQ Duck SAMQKNLECPICLDVVKEPVSTKCDHIFCRFCMYKLLSKKKKGVVQCPLCKTEVTKRSLT **** *******:::*************:*** ***.::* * ******..:***** Human ESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKK--ENNSPEHLKDEVSIIQSMGYRN Cat ESTRFSQLVEELLNIIHAFELDTGLQFANSYNFSKK--ENNSPEHLKEEVSIIQSMGYRN Elephant ESTRFSQLVEELLKIIRAFELDTGLQFANSYNFAKK--ENNSPEHVKEEVSIIQSMGYRN Black ESTRFSQLVEELLKIIHAFQLDTGLQCN----------------------AVIMGMRYHS Mouse GSTRFSQLAEELLRIMAAFELDTGMQLTNGFSFSKK--RNNSCERLNEEASIIQSVGYRN Duck ENSRFKQLIEGLLEAIHAFELDTGVKFLNSHRLPKSSTEATAAKLPCNESSVIQSKGFRN .:**.** * **. : **:****:: ::* . ::. Human RAKRLLQSEPENPSLQETSLSVQLSNLGTVRT-LRTKQRIQPQKTSVYIELGSDSSEDTV Cat RAKRLRQSEPENP-NLETSLSVQLSNFGIVRS-LRTKQQIQPQNKSVYIELGSDSSEDTV Elephant RAKRLRQSEPENPTLQETSLNAQLSNFGIVRS-LRTKQHTQLQNKSVYIELGSDSSEDTV Black QPQRFYTEPANNCLTQETSLSVPLSNFGIVRT-LRTKQRIQPQKKSVYIELGSDSSEDMV Mouse RVRRLPQVEPGNATLKDS-LGVQLSNLGIVRS-VKKNRQTQPRKKSVYIELDSDSSEETV Duck RKRSAKENEQENCSLQGANVDAQRADISVKRCSLRNRNQKHDLEKGVFIELGTDSSEELC : : * : :.. :::. * ::...: : :..*:***.:****: Human NKATYCSVGDQELLQITPQGTRDE--------------------ISLDSAKKG------- Cat NKASYFSAEDDELLGITPQGARAE--------------------ASLNPAKKASCEFSE- Elephant NKESYYSVVDQELLHIAPQGAKAE--------------------ASLDPTKKAACEFSEK Black NKATYCSVGDQELLQITPQGTRDE--------------------TSLDSAKKAACEFSEK Mouse TKPGDCSVRDQELLQTAPQEAGDE--------------------GKLHSAEEAACEFSEG Duck KQAGNTGLGNKEVVQISSEEKLEEPKSPEKGNEYSCNTRPTELRAKEIILPNVIGESDFS .: . :.*:: :.: * . : Human ------------------------------------------------------------ Cat DITNIEHRQPSNKDLTATEKHATEKHPEKYQGISVSNLHVEPCATNTRASSLQHENSSLL Elephant DITNLEYRQSSNKDLNTIKKHAAEKHPEKYRGISVSNLNVEPCGTNTHASSLQHKNSSLL Black DITNTEHHQSSNNDLNTTEKHATERHPEKYQGSSVSNLHVEPCGTNTHASSLQHENSSLL Mouse --IRNIEHHQCSDDLNPTENHATERHPEKCQSISISNVCVEPCGTDAHASSLQPETSSLL Duck KEALSKKSMQSVAEHANPDQVNEMECQSSPLNILSPDLLTERCDRANNASPLKDGDTSLS Human ------------------------------------------------------------ Cat LTKGRMNVEKAEFCNESKQPGVARSQQSRWAESKETCNDRQTPSTEKKVVLNADPLCRRK Elephant LTKDRMNVEKAEFCNKSKQPGLARSQQSRWAESKETCNDRQTPSTEKKVDVNADPLYERK Black LTKDRMNVEKAEFCNKSKQPGLARNQHNRWTGSKETCNDRQTPSTEKKVDLNADTLYERK Mouse LIEDRMNAEKAEFCNKSKQPGIAVSQQSRWAASKGTCNDRQVPSTGEKVGPNADSLSDRE Duck KNAEETGAEQTQCSNKSQEFDLEDSSENRPGKSKEIDAVVQSVEDVEMCESENNSEKESP Human ------------------------------------------------------------ Cat -LSKQKSPCSDSPRDSQDVPWITLNSSIQKVNEWFSRSDEMLTSDDSHDEGSESNPGVA- Elephant EVNKQKPPRSENLRDTQDIPWITLNSSIQKVNEWFFRSDGLDVLNDEGPESSAEVAG--- Black ERNKQKLPCSENPRDTEDVPWITLNSSIQKVNEWFSRSDELLSSDDSHDGGSESNAKVAD Mouse KWTHPQSLCPENSGATTDVPWITLNSSVQKVNEWFSRTGEMLTSDSASARRHESNAEAAV Duck -LEKLLQPETPHCPTLHQVSRKRLKRSIQKVNEWFSKSDEILSSNSSHDDPAEASDVSG- Human ------------------------------------------------------------ Cat GAEVPNVVDGYSGSSEKIDLMASDPHDALICESERVHTKPVESNIEDKIFGKTYRRKASL Elephant --ALEVPNEVHSNSSEKIDLMASDLHGALICESERVPSKPAESNIEDKIFGKTYRRKAGM Black VLDVLNEVDEYSGSSEKIDLLASDPHEPLICKSERVHSSSVESNIEDKIFGKTYRRKANL Mouse VLEVSNEVDGGFSSSRKTDLVTPDPHHTLMCKSGRDFSKPVEDNISDKIFGKSYQRKGSR Duck ---EGDALSDKDSCISEKTSPVVGFMETTVSEGNKRWSKEKTDNIKDKIFGKTYERERKS Human ------------------------------------------------------------ Cat PNLSHTSEDLIIGACAIEPQITQAYPLTNK--TKRKRRSTAGLHPEDFIKKVDLAIVQKT Elephant SHLSHITEDLIMGAIASEPQIAREHPFTNK--LKRKRRTSG-LHPEDFIKKVDLAVVQKI Black PNLSHVTENLIIGALVTESQIMQERPLTNK--LKRKRRTTSGLHPEDFIKKADLAVQKTP Mouse PHLNHVTEIIG--TFITEPQITQEQPFTNK--LKRKRSTS--LQPEDFIKKADSAGVQRT Duck NPPIIMREILPTTKKEDVPAVQECLNNSRKDSLKRKRKPVRHLQPEDFIKKKDVEEAGGC Human ------------------------------------------------------------ Cat PEKLIEGTDRIGQNGHVMNRTNNGPENGTKGDYVQKEKNAKPTESLEKESAFRTKAEPIS Elephant PEKINQETDHVEQNGQVMNIANGGRENETKGDYVQKEKNAIPTESLAKESAFRTKAEPIS Black -EMINQGTNQMEQNGQVMNITNSAHENKTKGDSIQNEKNPDPIESLEEESAFKTKAEPIS Mouse PDNINQGTDLMEPNEQAVSTTSNCQENKIAGSNLQKEKSAHPTESLRKEPASTAGAKSIS Duck PQSVNTCLG----DAEKKKCEESSALKENPPPEKRRGSTLAEFEEGASMSKNATEKVTCE Human ------------------------------------------------------------ Cat SNISNMESELNSHSSRAPKENRLRRKSSTRHMRALELVVNRNPSPPDHTELQIDSCSSSE Elephant SSISNMELELNMHNSKAPKKNRLRRKSSTRHIHALELVVNRNPSPPTHTELQIDSWSSSE Black SSINNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRNLSPPNCTELQIDSCSSSE Mouse NSVSDLEVELNVHSSKAPKKNRLRRKSSIRCALPLEP-ISRNPSPPTCAELQIDSCGSSE Duck HSGELELNNSDQKSTKNPSSAAKRCRRSTRTMCALQLVVDKNSASPDPAETLIESYPSSE Human ------------------------------------------------------------ Cat EMVKRKSSEQMLVRHSKTLQLVENKEPAPGAKKRNKPSKQINKRLASNTFPELNLTNIPG Elephant E-TKKKSSEQKPIRHNRNLQLMKNQETATGAKKSNKPKEQISKRHGADSYPELHLTTTAG Black E-IKKKNYNQMPVRHSRKLQLMEDKESATGAKKSNKPNEQTSKRHASDTFPELKLTKVPP Mouse E-TKKNHSNQQPAGHLREPQLIEDTEPAADAKK-NEPNEHIRKRRASDAFPEEKLMNKAG Duck EPRNADSEQRQVRRSRRLQLLSEEMTKETAKRAVIKEASKYDGDQEGPVFGVEKTVLVRD Human ------------------------------------------------------------ Cat VFTNCSSSNTLQEFVNPGIQREELEESRGTIHVSDRTRDPKALVSSGGRSLQTERSVEST Elephant FITKCSSSDNLQEFVNPSLQGEKTEENLETIQVSNITKEPKDLVLNGGRDLQTKKSIEST Black SFTNCSNTSELKEFVNPSLSREEKEEKLETVKVSNNAKDPKDLMLSGERVLQTERSVESS Mouse LLTSCSSPRKSQGPVNPSPQRTGTEQLETRQMSDSAKELGDRVLGGEPSGKTTDRSEEST Duck SECKDLCEQQDISSCKPLTNLESADLEANAIQMSPKNSSETAKTGKRLFNPAPSHQHSDL Human ------------------------------------------------------------ Cat SISLVPDADYGTQDSISLLEADTLGKAKTAPNQRVSLCAAIENPKEAIRGCSKDTRNGTE Elephant NISVIPDTVYGTQDSVSLLGADTPGKAKTAPNRCASQCTAIENPSELTNSCPKDTRNDTE Black SISLVPGTDYGTQESISLLEVSTLGKAKTEQNKCVSQCAAFENPKELIHGCSEDTRNDTE Mouse SVSLVSDTDYDTQNSVSVLDAHTVRYARTGSAQCMTQFVASENPKELVHGSNNAGSGTEG Duck NSSSPRAGSEEGEVLGDPFLHQSPSKTVVQTASHLPEGKRAESCPTLPQDKEHSTQNVPE Human ------------------------------------------------------------ Cat SFTDPLRREDNHTQETSIEMEENELDTQCLYNTFKVSKRQSFALFSNPGNPEKECATAWA Elephant GFKDLLRCEASHIQETCIEIEESELDTQYLQSTFKVSKRQSFALFSN---PEKKCATICA Black GFKYPLGREVNHSQETSIEIEESELDTQYLQNTFKVSKRQSFALFSNPGNPEEECATFSA Mouse --LKPPLRHALNLSQEKVEMEDSELDTQYLQNTFQVSKRQSFALFSKPRSPQKDCAHSVP Duck GLRTEKSPMAKNVSELAKESEDSEQDTQYLRNIFRNSKRLSFSLYPAPRKACEIDDVASE Human ------------------------------------------------------------ Cat RSTSLRKQSPKVALEYEQKEENQGKKQSEIKHVQAVRATAGFSAVSQKVEKPGDYAKCSI Elephant HSKSLRKQSPKVTLVCGEKEENQGNREPKIKHEQAVHMPTGYPEACQK-EKPSDYTKYSI Black HSKSLKKQSPKVTSECEQKEENQGKKESNIKPVQTVNITAGFPVVCQK-DKPVDNAKCSI Mouse SKELSPKVTAK-----GKQKERQGQEEFEISHVQAVAATVGLPVPCQE---GKLAADTMC Duck KLKMSYADQVEKRHSKYFNTENLQKEKTAENLSNVCEKEKMKNCESACASPVSCTVSNAE Human ------------------------------------------------------------ Cat KGVPGLCQSSQFRGNETELFIANNHDISKNPYHIPPLSPIRSSVNAVCKKNLSEEKFEQR Elephant KGVSGLCQSSQFRGSESELITADGHGISQNPDQIPSLSPTRSSVKTKCKTNLLEERFEEH Black KGGSRFCLSSQFRGNETGLITPNKHGLLQNPYHIPPLFPVRSFVKTKCKKNLLEENSEEH Mouse DRGCRLCPSSHYRSGENGLSATGKSGISQNSHFKQSVSPIRSSIKTDNRKPLTEGRFERH Duck CACSGDCQDVSQVANQGNLMTPVRMGAASSEGKNRPQKGERGSEKTASTDAGAESVLRQN Human ------------------------------------------------------------ Cat SMSPERAVGNESVIQSTVNTISQNNIRENTFKEVSSSSVNEVGSSANEVGSSINEVGSSG Elephant TISLERAMRNENVIQSTVSTVSQNNIRESASKEASSSSIN-------EVCSSINEVGSSG Black SVSPERTVGNENIIPSTVSTISHNNIRENAFKEASSSNINEVGSSTNEVGSSINEVGSSD Mouse TSSTEMAVGNENILQSTVHTVSLNNRGNACQEAGSGS---------------IHEVCSTG Duck PVKSSKSQSVQSNTEEHVFQRTDLNTVSETCFSSESDQ------------------AEKA Human ------------------------------------------------------------ Cat ENIQTELGRNRGPKLSAMLRLGLMQPEVYEQSLPISNCKCPEMKRQGENEGVVQSVNADF Elephant ENIQAEISRKRGPKLNAVLRLGLMQPEVYKQSLPISDCKHPGIKTQGENEGVVQAVNTDF Black ENIQAELGRNRGPQLNAVLRLGLLQPEVCKQSLPISNCKHPEIKKQ-EHEEVVQTVNTDF Mouse DSFPGQLGRNRGPKVNTVPPLDSMQPGVCQQSVPVSDKYLEIKKQE------GEAVCADF Duck EFVDSEGPVLCFQPGSMARPTACQQRSAEFNCKDAEKRRSKRRRLIGDEEPATQTASTGV Human ------------------------------------------------------------ Cat SPCLISDNVEQPMESSRASQVCSETPNDLLNGDEIKGKISFAESAKERS-AVFGKSVQKG Elephant SPCLISDNLEQPVGTSRASQVCSETPDDLLDDDEIKENISFAENGIKER-SVFIKDDQRR Black SPCLISDNLEQPMGSSHVSEVCSETPDDLLDDGEIKEDTSFAENDIKESSAVFSKSVQRG Mouse SPCLFSDHLEQSMSG-KVFQVCSETPDDLLDDVEIQGHTSFGEGDIMERSAVFNGSILRR Duck PECLVAETLEETFKG-GCSDTSETPDGLMCSDDDSEENVSFCEAPRKDSSAVFVKSDLVK Human ------------------------------------------------------------ Cat EFRRSPSPLDHTHLAQGHQTETRKLESSEENVSSEEEELPCFQHLLFGKVTSIPSQSTSH Elephant EFRRNPSPLSHSGLAQGCLRGARELEPSQENISSEDEELPCFQHLLFGEVTNIPSQSTRH Black ELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSEDEELPCFQHLLFGKVSNIPSQTTRH Mouse ESSRSPSPVTHASKSQSLHRASRKLESSEESDSTEDEDLPCFQHLLSRISN--TPELTRC Duck ERDHGGVGSQPRSTGIQRARRRAQKLQSSSEESSEDEDLPCFQALIFGKSASTPLQTNKQ Human ------------------------------------------------------------ Cat NAIATEGLSKKTEANLESLKDSLHDCSNQVTSAKESQEHHLSEEARCSGSLFSSQCSASE Elephant NADAIQCLSKNTEENLGSVQNSINDCSNQVTSAKASQERHLSEEVRCSSSLFSSQCSVME Black STVATECLYKNTEENLLSLKNSLTDCSNQVILAKASQEHHLSEETKGSGSLFSSQCSELE Mouse SSAVTQRMPEKAEGTQAPWKGSSSDCNNEVIMIEASQEHQFSEDPRCSGSMFSSQHSAAQ Duck VTSALGSSGNPNTLPRGGSREDKVQKTPEAALSNGCASP--SQESECSVNLFSSQSNVSE Human ------------------------------------------------------------ Cat DSAVNTNTQDPFLMFDPPSKQVRHQSENV-DVLSDEELVSYDDEREPGLEEDNHEEEQSV Elephant DLATNTNTQDPFLMFGSPSKQARHQSENQEVVLSDEELVSDEYKRGPGLEEDNHQDDQSV Black DLTANTNTQDPFLIGS--SKRMRHQSESQGVGLSDKELVSDDEERGTGLEEDN-PEEQSV Mouse GSTANANSQDSNFIPP--SKQRSHQCGNEEAFLSDKELISDNEEMATCLEEDNDQEEDSI Duck ESADGAQEPKKPLLQVHASKQQSNVSESKETLQSCAGRLKRRKTNSKDECQED------- Human -----EAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQH Cat DSNLGEAASGYENETSLSEDCSGVSSQSDILTTQQRDTMQDNLIKLQQEMAELEAVLEQH Elephant DSNLDEVASGYESESSLSEAGSGPSSQSDILTTQQRDTMQDNLIKLQQEMAELEAVLEQQ Black DSDLGEAASGYESETSVSEDCSRLSSQSEILTTQQRDTMQDNLIKLQQEMAVLEAVLEQH Mouse IPD--SEASGYESETNLSEDCS----QSDILTTQQRATMKYNLIKLQQEMAHLEAVLEQR Duck --PNLGEASGYDSETSHVEDSCGPFSQGEILSTQQKNAMQNNLKKLQQEMAALEAVLQEH *** :.*:. * . *.:**:***: :*: ** ******* *****::: Human GSQPSNSYPSIISDSSALEDLRNPEQSTS------EKAVLTS---------QKSSEYPIS Cat ESQPSNSSPALIRDSYAPEDLFNLEQNTS------EKAVLTS---------EKSSDCLIS Elephant GSQPSNSSPSLLAGSRAPKNLLNPEQNTS------EKGSISTS--------EKSNEHPVS Black GSQPSNSYPSIVTDSSALEDLRNPEQSTS------EKGICFLIPFKAVLTSQKSSEYPIS Mouse GNQPSGHSPSLLADPCALEDLPDLEPNMSGAAILTSKN---------------INENPVS Duck GSQDSEPLPACRELLGPRSERTLAVEQTR------QRGAESTS--------EGSLENHKC .* * *: . .: . .: : . Human QNPEGLSADKFEVSADS-STSKNKEPGVERSSPSKCPSLDDR-WYMHSCSGSLQNRNYPS Cat QNPESLSADKFQVSLDS-STSKTKEPGMRRSSPSQSRLLDTR-QCVRSHSSSLQSTNCLS Elephant ENPEGLSADKFRPSLDS-ATSKNKDPGMERSSPSKFQLLDNNRWDVHSHSRSLQNGNRLS Black QNPEGFSADKFKVSADS-STSKNKEPGVERSSPSKCQSLEDR-WYVHSSSGSLQNGNYPS Mouse QNLKSACDDKFQLQHLEGPTSGDDESGMGRPSPFKSPLAGSR-GSAHGCSRHLQKRNSPS Duck STSKGFSADEVHVIPSDSSNSEVKELEIERSPELPYPSLKSS----WSKRPDLEEDLRHD .. :. . *:.. . ..* .: : *.. . *:. . Human QEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRA Cat QKELIKAVDGEELQLTKSEPRDFMEQSYLPRQDLEGTPYFESGISLFSDDPESDFSVDRA Elephant QEELVKIVDVEEQQVEKSEARGLIEQPYLPLQDLEGTPCLESGVSLFSDDPESDPSEDRA Black QEELIKVVDMEEQQLEKSGPHNLMEPSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRA Mouse QEELLQPAGSEAS----SEPHNSTGQSCLPRRELEGTPYLGSGISLFSS---RDPESESP Duck SVVENKAPQETKPVHEAVQEYSQSEEENADEKSGTKLNKASVLNDLCGNVTRSPHASSSF . : . :. .* .. . Human PESARVGNIPSSTSALKVPQLKVAESAQSPAAAHTTDTAGYNAMEESVSREKPELTASTE Cat PEPVHVSSMPTSTSALKLPQFQVEESAKSTAAVHITNTAGYNKREDIEGKERPEMMSSTK Elephant PDPAHVCSSPASSSALKLPQFQVAESASDSADAHTNNTTAYNAREESVNKETPGVTSSAE Black LESAHVGSIPSSTSALKVPQWQVAESAQSPAAAHNTNTAEYNAMEESVSREKPKLTASTE Mouse KEPAHIGTTPASTSALKIPQGQVAFRSAAAAGADKAVVG-------IVSKIKPELTSSEE Duck VKPPHLQTAEATNSSAVVQN----DNKSSSQGCKSKRSVCFPTSVLHNAYGKENATNPVV .. :: . ::.*: : : . . . Human RVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYF Cat RVNKRISMVASGLTPKELVLVQKFARRHQITLTNVISEETTHVIMKTDAEFVCERTLKYF Elephant RANRRASMVASGLTQKEFMLVHKFARKHHCTLTNQITEETTHVIMKTDADFVCERTLKYF Black RVNKRMSLVVSGLTPEEFMLVYKFARRYHIALTNLITEETTHIVMKTGIPRTFT------ Mouse RADRDISMVVSGLTPKEVMTVQKFAEKYRLTLTDAITEETTHVIIKTDAEFVCERTLKYF Duck TNRKKMSIVASGLKQSEHMVVQKFARKTQSTFSNHITDGTTHVIMKTDKELVCERTLKYF : *:*.***. .* : * ***.: : :::: *:: ***:::**. . Human LGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGL Cat LGIAGGRWVVSYFWVTQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESRDRKIFGGL Elephant LGIAGGKWVVSYFWVTQSIKERKMLDECDFEVRGDVVNGRNHQGPKRAREFQDRKIFKGL Black ---------------------EYFAFAHDFEVRGDVVNGRNHQGPKRARESPDRKIFRGL Mouse LGIAGGKWIVSYSWVVRSIQERRLLNVHEFEVKGDVVTGRNHQGPRRSRESRE-KLFKGL Duck LGIAGRKWVVSYQWVVQSFKEGMILDEENFEVRGDVINGRNHQGPKRARQSLTEKIFKDF : :***:***:.*******:*:*: *:* .: Human EICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAI Cat EICCYGPFTNMPTDQLEWMVRLCGASVVKRPSLFTLSKATHPVVVVQPDAWTEDSGFHAI Elephant EICCYGPFTNMPTDQLEWMVQLCGASVVKEPLSFTLGTGTHPIVVVQPDAWTEDSGFHAI Black EICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTVSICCPVRE--------EGhHTI Mouse QVYCCEPFTNMPKDELERMLQLCGASVVKELPSLTHDTGAHLVVIVQPSAWTEDSNCPDI Duck EICCYGPFTDMTTGHLEWMVELCGASVVKQLHLFTHKMNSTAVVVVQPDAWMEGTSYKAI :: * ***:*....** *:.********. :* : : * Human GQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHYORTHOLOGUS Cat GQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPRATADLSQPCV--- Elephant GQLCEAPVVTREWVLDSVALYQRQELDTYLIPQIPHSHY---------- Black GQMCEAPVVTREWVLDSVALYQCQELDAYLIPQIPHSHY---------- Mouse GQLCKARLVMWDWVLDSLSSYRCRDLDAYLVQNITCDSSEPQDSND--- Duck QQKNNVAVVTREWVLDSVACYECQELDAYLVS----------------- * :. :* :*****:: *. ::**:**: ACTIVITY# 3 Description Scientific Name Query Cover E value Per. Ident Accession breast cancer type 1 susceptibility protein isoform 105 [Homo sapiens] Homo sapiens 100% 0.0 100.00% NP_- breast cancer type 1 susceptibility protein homolog isoform X11 [Pan troglodytes] Pan troglodytes 100%-% XP_- breast cancer type 1 susceptibility protein isoform X10 [Pongo abelii] Pongo abelii 100% 0.0 97.78% XP_- breast cancer type 1 susceptibility protein homolog isoform X9 [Gorilla gorilla gorilla] Gorilla gorilla gorilla 100% 0.0 93.55% XP_- breast cancer type 1 susceptibility protein homolog isoform X6 [Macaca mulatta] Macaca mulatta 100% 0.0 94.17% XP_- Homo sapiens >NP_- breast cancer type 1 susceptibility protein isoform 105 [Homo sapiens] MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITK RSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQS EPENPSLQETSLSVQLSNLGTVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQ GTRDEISLDSAKKGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHG SQPSNSYPSIISDSSALEDLRNPEQSTSEKAVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPG VERSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTP YLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQSPAAAHTTDTAGYNAMEESV SREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLK YFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPF TNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSV ALYQCQELDTYLIPQIPHSHY Pan troglodytes >XP_- breast cancer type 1 susceptibility protein homolog isoform X11 [Pan troglodytes] MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITK RSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQS EPENPSLETSLSVQLSNLGTVRTLRTKQRIQPQKKSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQG TRDEISLDSAKKAACEFSETDVTNTEHHQPSNNDLNTTEKRATERHPEKYQGEAASGCESETSVSEDCSG LSSQSDILTTQQRDTMQDNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLQNPEQSTSEKAV LTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYPS QEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDKAPESAHVGNIP SSTSALKVPQLKVAESAQSPAAAHTTNTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFML VYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDF EVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGV HPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY Pongo abelii >XP_- breast cancer type 1 susceptibility protein isoform X10 [Pongo abelii] MDLSAVRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITK RSLQESTRFSQLVEELLKIICAFQLDTGLQYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQS EPENPSLQETSPSVQLSNLGTVRTLRTKQRIQPQKKSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQ GTSDEISLDSAKKGEAASGYESETSVSEDCSGLSSQSDILTTQQRDTMQDNLIKLQQEMAELEAVLEQHG SQPSNSYPSIISDSSALEDLRNPEQSTSEKAVLTSQKSSEYPISQNPEGLSADKFEVSADSSTNKNKEPG VERSSPSKCPSLDDRWYMHSCSGSLQNGNYPSQEELIKVVDVEKQQLEESGPHDLTEPSYLPRQDLEGTP YLESGISLFSDDPESDPSEDRAPESAHVGSIPSSTSALKVPQLKVAESAQSPAAAQTTNTAGYNAMEESV SREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLK YFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPF TNMPTDQLEWIVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSV ALYQCQELDTYLIPQIPHSHY Gorilla gorilla gorilla >XP_- breast cancer type 1 susceptibility protein homolog isoform X9 [Gorilla gorilla gorilla] MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITK RSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSTGYRSRAKRLLQS EPENPSLETGLSVQLSNLGTVRTLRTKQRIQPQKKSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQG TRDEISLDSAKKAACEFSERDVTNTEHHQPSNNDLNTTEKRATERHPEKYQGEAASGCESETSVSEDCSG LSSQSDILTTQQRDTMQDNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKAV LTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYPS QEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESAHVGNIP SSTSALKVPQLKVAESAQSPAAAHTTNTAGYHAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFML VYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKEGKMLNEHDF EVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGV HPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY Macaca mulatta >XP_- breast cancer type 1 susceptibility protein homolog isoform X6 [Macaca mulatta] MDLSAVRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCRFCMLKLLNQKKGPSQCPLCKNDITK RSLQESTRFSQLVEELLKIIHAFQLDTGLQFANSYNFAKKENHSPEHLKDEVSIIQSMGYRNRAKRLLQS EPENPSLQETSLSVPLSNLGIVRTLRTKQQIQPQKKSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQ GTRDETSLDSAKKGEAASGYESETSVSEDCSRLSSQSEILTTQQRDTMQDNLIKLQQEMAELEAVLEQHG SQPSNSYPSIITDSSALEDLRNPEQSTSEKAVLTSQKSSEYPINQNPEGLSADKFEVSADSSTSKNKEPG VERSSPSKCQSLEDRWYVHSSSGSLQNGNYPSQEELIKVVDVETQQLEKSGPHDLMEPSYLPRQDLDGTP YLESGISLFSDDPESDPSEDRAPESAHVGSIPSSTSALKVPQWQVAESAQSPAAAHNTNTAGYNAMEESV SRENPKLTASTERVNKRMSLVVSGLTPEEFMLVYKFARRYHIALTNLISEETTHVVMKTDAEFVCERTLK YFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESPDRKIFRGLEICCYGPF TNMPTDQLEWMVQLCGASVVKELSSFTLGTGFHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSV ALYQCQELDTYLIPQIPHSHY CLUSTAL 2.1 multiple sequence alignment XP_- MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQ XP_- MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQ NP_- MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQ XP_- MDLSAVRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQ XP_- MDLSAVRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCRFCMLKLLNQKKGPSQ *****:**************************************:*************** XP_- CPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKD XP_- CPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKD NP_- CPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKD XP_- CPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLQYANSYNFAKKENNSPEHLKD XP_- CPLCKNDITKRSLQESTRFSQLVEELLKIIHAFQLDTGLQFANSYNFAKKENHSPEHLKD ****************************** ********::***********:******* XP_- EVSIIQSMGYRNRAKRLLQSEPENP-SLETSLSVQLSNLGTVRTLRTKQRIQPQKKSVYI XP_- EVSIIQSTGYRSRAKRLLQSEPENP-SLETGLSVQLSNLGTVRTLRTKQRIQPQKKSVYI NP_- EVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLGTVRTLRTKQRIQPQKTSVYI XP_- EVSIIQSMGYRNRAKRLLQSEPENPSLQETSPSVQLSNLGTVRTLRTKQRIQPQKKSVYI XP_- EVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVPLSNLGIVRTLRTKQQIQPQKKSVYI ******* ***.************* **. ** ***** ********:*****.**** XP_- ELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKAACEFSETDVTNTEHHQ XP_- ELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKAACEFSERDVTNTEHHQ NP_- ELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAK------------------ XP_- ELGSDSSEDTVNKATYCSVGDQELLQITPQGTSDEISLDSAK------------------ XP_- ELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDETSLDSAK------------------ ******************************** ** ****** XP_- PSNNDLNTTEKRATERHPEKYQGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQDN XP_- PSNNDLNTTEKRATERHPEKYQGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQDN NP_- ---------------------KGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHN XP_- ---------------------KGEAASGYESETSVSEDCSGLSSQSDILTTQQRDTMQDN XP_- ---------------------KGEAASGYESETSVSEDCSRLSSQSEILTTQQRDTMQDN :****** *********** *****:***********.* XP_- LIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLQNPEQSTSEKAVLTSQKSSEY XP_- LIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKAVLTSQKSSEY NP_- LIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKAVLTSQKSSEY XP_- LIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKAVLTSQKSSEY XP_- LIKLQQEMAELEAVLEQHGSQPSNSYPSIITDSSALEDLRNPEQSTSEKAVLTSQKSSEY ******************************:********:******************** XP_- PISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYP XP_- PISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYP NP_- PISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYP XP_- PISQNPEGLSADKFEVSADSSTNKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNGNYP XP_- PINQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCQSLEDRWYVHSSSGSLQNGNYP **.*******************.*************** **:****:**.****** *** XP_- SQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDK XP_- SQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDR NP_- SQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDR XP_- SQEELIKVVDVEKQQLEESGPHDLTEPSYLPRQDLEGTPYLESGISLFSDDPESDPSEDR XP_- SQEELIKVVDVETQQLEKSGPHDLMEPSYLPRQDLDGTPYLESGISLFSDDPESDPSEDR ************ ****:****** *.********:***********************: XP_- APESAHVGNIPSSTSALKVPQLKVAESAQSPAAAHTTNTAGYNAMEESVSREKPELTAST XP_- APESAHVGNIPSSTSALKVPQLKVAESAQSPAAAHTTNTAGYHAMEESVSREKPELTAST NP_- APESARVGNIPSSTSALKVPQLKVAESAQSPAAAHTTDTAGYNAMEESVSREKPELTAST XP_- APESAHVGSIPSSTSALKVPQLKVAESAQSPAAAQTTNTAGYNAMEESVSREKPELTAST XP_- APESAHVGSIPSSTSALKVPQWQVAESAQSPAAAHNTNTAGYNAMEESVSRENPKLTAST *****:**.************ :***********:.*:****:*********:*:***** XP_- ERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKY XP_- ERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKY NP_- ERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKY XP_- ERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKY XP_- ERVNKRMSLVVSGLTPEEFMLVYKFARRYHIALTNLISEETTHVVMKTDAEFVCERTLKY ********:******************::**:*****:********************** XP_- FLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRG XP_- FLGIAGGKWVVSYFWVTQSIKEGKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRG NP_- FLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRG XP_- FLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRG XP_- FLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESPDRKIFRG ********************** ***************************** ******* XP_- LEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHA XP_- LEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHA NP_- LEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHA XP_- LEICCYGPFTNMPTDQLEWIVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHA XP_- LEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGFHPIVVVQPDAWTEDNGFHA *******************:********************.******************* XP_- IGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY XP_- IGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY NP_- IGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY XP_- IGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY XP_- IGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY **************************************** ACTIVITY#4 EXON 1 BY ARSHI AGAGGTGCCAGGAATATGTCTGGGAAGGGGAGGAGACAGGATTTTGTGGGAGGGAGAACT TAAGAACTGGATCCATTTGCGCCATTGAGAAAGCGCAAGAGGGAAGTAGAGGAGCGTCAG TAGTAACAGATGCTGCCGGCAGGGATGTGCTTGAGGAGGATCCAGAGATGAGAGCAGGTC ACTGGGAAAGGTTAGGGGCGGGGAGGCCTTGATTGGTGTTGGTTTGGTCGTTGTTGATTT TGGTTTTATGCAAGAAAAAGAAAACAACCAGAAACATTGGAGAAAGCTAAGGCTACCACC ACCTACCCGGTCAGTCACTCCTCTGTAGCTTTCTCTTTCTTGGAGAAAGGAAAAGACCCA AGGGGTTGGCAGCAATATGTGAAAAAATTCAGAATTTATGTTGTCTAATTACAAAAAGCA ACTTCTAGAATCTTTAAAAATAAAGGACGTTGTCATTAGTTCTTTGGTTTGTATTATTCT AAAACCTTCCAAATCTTAAATTTACTTTATTTTAAAATGATAAAATGAAGTTGTCATTTT ATAAACCTTTTAAAAAGATATATATATATGTTTTTCTAATGTGTTAAAGTTCATTGGAAC AGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTA TGCAGAAAATCTTAGAGTGTCCCATCTGGTAAGTCAGCACAAGAGTGTATTAATTTGGGA TTCCTATGATTATCTCCTATGCAAATGAACAGAATTGACCTTACATACTAGGGAAGAAAA GACATGTCTAGTAAGATTAGGCTATTGTAATTGCTGATTTTCTTAACTGAAGAACTTTAA AAATATAGAAAATGATTCCTTGTTCTCCATCCACTCTGCCTCTCCCACTCCTCTCCTTTT CAACACAAATCCTGTGGTCCGGGAAAGACAGGGACTCTGTCTTGATTGGTTCTGCACTGG GGCAGGAATCTAGTTTAGATTAACTGGCATTTTGGCTTTTCTTCCAGCTCTAAAACAAGC TCCATCACTTGAAATGGCAAAATAAAATCATGGATGAGGCCGAGGGCGGTGGCTTATGCC TGTAATCCCAGCACTTTGGGAGGCCAAGGTGGTAGGATCACGAGGTCAGGAGATCGAGAC start len tm diff tm gc% any_th 3’_th hairpin seq LEFT PRIMER- - GGGTTGGCAGCAATATGTGA RIGHT PRIMER- - GTTCTCCATCCACTCTGCCT EXON 2 BY MEHREEN CACTGCACTCCAGCCTAGGTGACAGAGTGTGACCTTATCTTAAAAGTAATAATAATTAAA ATAATCTGGGGTAGGGGTGGATATGGGTGAAACAGCTTGGCCATGAGTTGATGGTTGTTG GACCAGGGTGATGGTCCATATAGTTCATTTTATTATTTTATTTACTTGAAATTTTGAAAT ACTTGAAATTTTCCATATTAAGTTAAAAAGGCATTTACAGTAAACAAAAAAAAGTTCTAG GAAGGAATTCAAAAGAAATATAAGCAGAAAATTTTGTCTTTATGGAGCTTAAAGATGAGA TGTGCACCCACAGTGATAGTGCAGAAAAATATATCACTGGAAATGAATTCGTACGAACTA TTATCAACTAATCTTTTAAATGCTGATGATAGTATAGAGTATTGAAGGGATCAATATAAT TCTGTTTTGATATCTGAAAGCTCACTGAAGGTAAGGATCGTATTCTCTGCTGTATTCTCA GTTCCTGACACAGCAGACATTTAATAAATATTGAACGAACTTGAGGCCTTATGTTGACTC AGTCATAACAGCTCAAAGTTGAACTTATTCACTAAGAATAGCTTTATTTTTAAATAAATT ATTGAGCCTCATTTATTTTCTTTTTCTCCCCCCCTACCCTGCTAGTCTGGAGTTGATCAA GGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAGTAAGTTTGAATGTGTTATGT GGCTCCATTATTAGCTTTTGTTTTTGTCCTTCATAACCCAGGAAACACCTAACTTTATAG AAGCTTTACTTTCTTCAATTAAGTGAGAACGAAAAATCCAACTCCATTTCATTCTTTCTC AGAGAGTATATAGTTATCAAAAGTTGGTTGTAATCATAGTTCCTGGTAAAGTTTTGACAT ATATTATCTTTTTTTTTTTTTTTGAGACAAAGTCTCGCTCTGTCGCCCAGGCTGGAGTGC AGTGGCATGATCTTGGCTCACTGCAACCTCCGCCCCCCGAGTTCAAGCGATTCTTCTACC TCAGCCTCCCAGGTAGCTGGGACTACAGGCACCCGCCACCATGCTTGGCTAATTTTTGTA CTTTTAGTAGAGATAAGGTTTCACCATATTGGCCAGGCTGGTCTCGAACTCCTGACCTTG TGATCCACCTGCCTCGGCCTCCCAAAGTTCTGGGATTACAGGCGTGAGCCACCACACCCG start len tm diff tm gc% any_th 3’_th hairpin seq LEFT PRIMER- GCTCACTGAAGGTAAGGATCG RIGHT PRIMER- - AAAGTCTCGCTCTGTCGC EXON 3 BY HAREEM AATTGTCTTGGTATTTATTATTGTTGAAAATCGCTTGATCACAGATGTATGTATGAGTTT ATTTCTGTACTGTCAATTCCATTTTATTGATGTATGTGTCTATTCTTATGCTATTACCAC ACTTTCTTGATTACTATAGCTTTGTGGTGAGGTGTTGAGATTTTAAACTAATTATAAGCA TCTTACATGAACTACTTACCGTTTATATTTGATTATGCAGCATGAAATAATTATGAATAT ATCATTAAATATGCCATATTAACTTTTATTAAGTTTTATGTGATCATAACAGTAAGCCAT ATGCATGTAAGTTCAGTTTTCATAGATCATTGCTTATGTAGTTTAGGTTTTTGCTTATGC AGCATCCAAAAACAATTAGGAAACTATTGCTTGTAATTCACCTGCCATTACTTTTTAAAT GGCTCTTAAGGGCAGTTGTGAGATTATCTTTTCATGGCTATTTGCCTTTTGAGTATTCTT TCTACAAAAGGAAGTAAATTAAATTGTTCTTTCTTTCTTTATAATTTATAGATTTTGCAT GCTGAAACTTCTCAACCAGAAGAAAGGGCCTTCACAGTGTCCTTTATGTAAGAATGATAT AACCAAAAGGTATATAATTTGGTAATGATGCTAGGTTGGAAGCAACCACAGTAGGAAAAA GTAGAAATTATTTAATAACATAGCGTTCCTATAAAACCATTCATCAGAAAAATTTATAAA AGAGTTTTTAGCACACAGTAAATTATTTCCAAAGTTATTTTCCTGAAAGTTTTATGGGAC ATCTGCCTTATACAGGTATTAGAAACTTACTGCCTTTCTCTAATGCTTCTAGTGTAAAAA CTTGCAGACTTATGTAAAGTAGGGCTGTATCGCCGTGCCCCCATTGTCTGTTAATCTTGT TTTTATATTTTTGATTGTGTTTCCTTTTCTTTTTTTTTTTTTTTTTAAGACAGGGTCCTG CTCTGTCACTGAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGTAGCCTCTGTCTCC CAGCCTCTTCCTGCCTTAGCCTCCCAAATAGCTGGGACTACAGGCACACGCTACCATGCC CGGCCAATTTTTGTATTTTTTGTAGAGATGAGGTTTTACCATGTTGCCCAGGCTGGTAAC TCCTGAGCTCAGGTGATCTGCCCACCTCGGCCTCCCAAAGTGCTGGGGTTCACAGGTGTG start len tm diff tm gc% any_th 3’_th hairpin seq LEFT PRIMER 139 20 58.61 - GCTTTGTGGTGAGGTGTTGA RIGHT PRIMER- 55.00 - ATAGCTGGGACTACAGGCAC EXON 4 BY MAIMOONA ACATAGTGAGACCCCATCACTACCAACAAAATAAATAAATAAATGTACATGGTGGCATAT GCCCATAGTCCTAGCTACTTGGGAGGCTATAGTGGGAGGATAGCTTGAGTACAGAAGTCT GAGGCTGCAGTGAGCTATGATTGTGGCACTGCATGCTAGCCTGGGCAATAGAGCAAGACC CTGTCTCTAAATTAAACAAAAAAAAAAGTACTCTAGTTTTCTATGCAATGCATTATATCT GCTGTGGATTTAGGGCAGTATTATATCAGATAATTTTAGGCATTTGGTAGGCTTAAATGA ATGACAAAAAGTTACTAAATCACTGCCATCACACGGTTTATACAGATGTCAATGATGTAT TGATTATAGAGGTTTTCTACTGTTGCTGCATCTTATTTTTATTTGTTTACATGTCTTTTC TTATTTTAGTGTCCTTAAAAGGTTGATAATCACTTGCTGAGTGTGTTTCTCAAACAATTT AATTTCAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAA ATCATTTGTGCTTTTCAGCTTGACACAGGTTTGGAGTGTAAGTGTTGAATATCCCAAGAA TGACACTCAAGTGCTGTCCATGAAAACTCAGGAAGTTTGCACAATTACTTTCTATGACGT GGTGATAAGACCTTTTAGTCTAGGTTAATTTTAGTTCTGTATCTGTAATCTATTTTTAAA AAATTACTCCCACTGGTCTCACACCTTATTTTATCAATCGTAAGGTGCACATTTTTCACA TCTTAACATCTCTGAAATTGGGAACATTTTACTATTGAGGGTGTGTCATTTGTTTAATTT GTGTGCTTTCTTTCTTAGTGATACAGAAAATAATAGTGCAACTTACATTGTTGGTGTCTT AGCTTTAGTGAAATACAGTATTGATAGGCAAATTTCTTAGTGTTAAGGTAGAAAACAAGG ACTCTAAATAACTTTGATGGTCTGTGTATTTGTTTTTGTTTCCTAGGAGTAAAATTTCCA GTTGATTTTTTAAAATTTGATTTTTAAAAAAAATCACAGGTAACCTTAATGCATTGTCTT start len tm diff tm gc% any_th 3’_th hairpin seq LEFT PRIMER- AAATCACTGCCATCACACGG RIGHT PRIMER- - ACTCAAGTGCTGTCCATGAA EXON 5 BY LALEENA GCGCCACTGCACTCCAGCCTGGGTGACAGAGCAGGACTCTGTACACCCCCCAAAACAAAA AAAAAAGTTATCAGATGTGATTGGAATGTATATCAAGTATCAGCTTCAAAATATGCTATA TTAATACTTCAAAAATTACACAAATAATACATAATCAGGTTTGAAAAATTTAAGACAACA GAAAAAAAAATTCAAATCACACATATCCCACACATTTTATTATTACTACTACTATTATTT TGTAGAGACTGGGTCTCACTCTGTTGCTTATGCTGGTCTTGAACTCCTGGCCTCAAGCAG TCCTGCTCCAGCCTCCCAAAGTGCTGGGATTATAGGCATGAGCTACCGCTCCCAGCCCCA GACATTTTAGTGTGTAAATTCCTGGGCATTTTTTCCAGGCATCATACATGTTAGCTGACT GATGATGGTCAATTTATTTTGTCCATGGTGTCAAGTTTCTCTTCAGGAGGAAAAGCACAG AACTGGCCAACAATTGCTTGACTGTTCTTTACCATACTGTTTAGCAGGAAACCAGTCTCA GTGTCCAACTCTCTAACCTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAAC CTCAAAAGACGTCTGTCTACATTGAATTGGGTAAGGGTCTCAGGTTTTTTAAGTATTTAA TAATAATTGCTGGATTCCTTATCTTATAGTTTTGCCAAAAATCTTGGTCATAATTTGTAT TTGTGGTAGGCAGCTTTGGGAAGTGAATTTTATGAGCCCTATGGTGAGTTATAAAAAATG TAAAAGACGCAGTTCCCACCTTGAAGAATCTTACTTTAAAAAGGGAGCAAAAGAGGCCAG GCATGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCAAAGTGGGTGGATCACCT GAGGTCGGGAGTTCGAGACCAGCCTAGCCAACATGGAGAAACTCTGTCTGTACCAAAAAA TAAAAAATTAGCCAGGTGTGGTGGCACATAACTGTAATCCCAGCTACTCGGGAGGCTGAG start len tm diff tm gc% any_th 3’_th hairpin seq LEFT PRIMER- TGGGTCTCACTCTGTTGCTT RIGHT PRIMER- - TTTGTGGTAGGCAGCTTTGG ACTIVITY#5 Primary Protein Structure Prediction PROTPARAM PROTSCALE ACTIVITY#6 Secondary Sturcture Prediction PSIpred By ARSHI Jpred 4 By HAREEM GOR-IV By MEHREEN PROTEUS2 By MAIMOONA PSIPRED By LALEENA ACTIVITY# 7 Tertiary Structure Prediction Swiss-model ACTIVITY# 8 Predict SNP THE END