Youakim Saliba

YOUAKIM SALIBA PHD PHYSIOLOGY & PATHOPHYSIOLOGY INTRODUCTION My research revolves around understanding the mechanisms underlying the pathophysiology of cardiac fibrosis and chronic kidney disease in order to develop new potential therapeutic strategies. Studying the implication of intracellular calcium in diverse cardiac and renal cellular functions is also pivotal along with developing animal models to study human disease. Author of over 40 articles in peerreviewed journals. EDUCATION Pierre and Marie Curie University - PhD, Physiology and Pathophysiology, 2012. Saint Joseph University of Beirut (USJ) - PhD, Physiology and Pathophysiology, 2012. University of Michigan Certificate of Competency in English, 2009. Saint Joseph University of Beirut (USJ) - Master's degree, Biology and Health - Option Physiology, 2008. University of Poitiers Specialized courses in Physiology for the completion of my Master's degree from the Saint Joseph University. Lebanese University - Maitrise ES Sciences en Biologie, Zoology/Animal Biology, 2007. CONTACT- inkedin.com/in/youakim-saliba researchgate.net/profile/ Youakim_Saliba WORK EXPERIENCE Since September 2012 Assistant Professor at the Faculty of Medicine and Research Scientist at the Physiology and Pathophysiology Research Laboratory, USJ. Laboratory seminars in the form of guided instruction. Co-supervision of doctoral students in Biological and Medical Sciences, Physiology and Physiopathology option, Faculty of Medicine, USJ (6 PhD students) and supervision of Master students in Functional Genomics and Proteomics, Faculty of Sciences, USJ (10 students) and Master 2 students in Biology – Health, Option Physiology, Faculty of Medicine, USJ (27 students). Management of the Physiology and Pathophysiology Research Laboratory, including writing projects for grants and acquiring commercial invoices from the suppliers of laboratory materials and equipment. Maintenance of the animal house for rodents (breeding, treatments and monitoring of lines) of the Physiology and Pathophysiology Research Laboratory, USJ. Since September 2016 Lecturer in Research Methodology, Master in Biological and Medical Sciences, Faculty of Medicine, USJ- Coordinator of the Physiology syllabus, Faculty of Sciences, USJ- Lecturer in Comparative Anatomy and Physiology, Human Physiology, and Organization of the Animal kingdom, Bachelor of Science in Life and Earth Sciences, Faculty of Sciences, USJ. Lecturer in Molecular Endocrinology, Master in Functional Genomics and Proteomics, Faculty of Sciences, USJ. SELECTED PUBLICATIONS & AWARDS Achkar A, Saliba Y, Fares N. Oxid Med Cell Longev. 2020 May 23;2020:-. Chacar S, Hajal J*, Saliba Y*, et al. Aging Cell. 2019 Apr;18(2):e12894. Beaini S*, Saliba Y*, et al. J Cell Physiol. 2019 Jun;234(6):-. Saliba Y, et al. Antioxid Redox Signal. 2019 Jun 1;30(16):-. Saliba Y, et al. J Am Soc Nephrol. 2015 Aug;26(8):1855-76. Saliba Y, et al. Cardiovasc Res. 2015 Mar 1;105(3):- Best scientific contribution awards from the Federation of European Physiological Societies - ANR Grant - Eiffel Excellence Scholarship and CEDRE fellowship. SKILLS English/French/Arabic language proficiency - Calcium imaging Echocardiography on small animals - Microsurgery - Animal models of human disease - Diverse primary cell isolation and culture - Use of lentiviruses, adenoviruses and AAV in vitro and in vivo - Vast array of biochemical and molecular techniques. Youakim SALIBA Curriculum Vitae 2020 Youakim SALIBA Born 06 August 1985 – Bteghrine – North Metn – Lebanon Tel: - Email:-https://scholar.google.com/citations?user=_TRGVEqUcsIC&hl=en&oi=ao https://www.researchgate.net/profile/Youakim_Saliba https://www.linkedin.com/in/youakim-saliba/ ACADEMIC TITLES – FORMATION 2008 – 2012 PhD in Physiology and Physiopathology (highest level of distinction), Pierre and Marie Curie UPMC Sorbonne University, Paris – and Saint Joseph University USJ, Beirut. Subject: «Identification of STIM1 partners in the normal and hypertrophied heart», under the supervision of Pr. Anne-Marie LOMPRÉ - Inserm UMR 956 ‘‘Physiology, Genetics and Cardiovascular Pharmacology’’, Pitié Salpêtrière (Recently: Inserm UMRS 1166, ICAN – Institute of CardioMetabolism and Nutrition) and Pr. Nassim FARES - Physiology and Pathophysiology Research Laboratory, USJ. 2009 Certificate of Competency in English with a High Pass – English Language Institute – The University of Michigan. 2007 – 2008 Master in Biology – Health, Option Physiology (highest level of distinction) – Faculty of Medicine – Saint Joseph University USJ – Beirut. Attendance of six specialized courses in Physiology and Pathophysiology at the University of Poitiers (Vascular signaling and pathogenesis, Calcium signaling, Glucose signaling, Inflammation, Channels gating, Molecular and cellular immunology, Embryology, Bacterial infections). Thesis subject: A pathophysiological and pharmacological approach of cardiorenal syndrome in the rat. 2003 – 2007 Master in Biology (mention good, 15.56/20) (rank 1st/19) - Faculty of Science 2, Lebanese University – Beirut, with an Award of Excellence for being the promotion major, and many honorary awards for the results of the 2nd and 3rd years (mention very good, 16.25/20) (Rank 2nd/79). Thesis subject: Influence of chemical releases from the Selaata plant on the meiobenthic populations of the coastal area of Batroun, under the supervision of Pr. Rita Mouawad. 2000 – 2003 Lebanese Baccalaureate, Life Sciences (mention good) – Saint Georges College, Basilian Chouerite Sisters – Khenchara. TEACHING AND RESEARCH EXPERIENCE Since September 2012  Assistant Professor at the Faculty of Medicine and Research Scientist at the Physiology and Pathophysiology Research Laboratory, USJ.  Laboratory seminars in the form of guided instruction.  Co-supervision of doctoral students in Biological and Medical Sciences, Physiology and Physiopathology option, Faculty of Medicine, USJ (6 PhD students) and supervision of Master 1 students in Functional Genomics and Proteomics, Faculty of Sciences, USJ (10 students) and Master 2 students in Biology – Health, Option Physiology, Faculty of Medicine, USJ (27 students).  Physiology and Pathophysiology Research laboratory management including writing projects for grants and acquiring commercial invoices from the suppliers for laboratory materials.  Maintenance of the animal house for rodents (breeding, treatments and monitoring of lines), of the Physiology and Pathophysiology Research Laboratory, USJ. Since September 2016  Lecturer in Research Methodology, Master in Biological and Medical Sciences, Faculty of Medicine, USJ-  Lecturer in Comparative Anatomy and Physiology, Bachelor of Science in Life and Earth Sciences, Faculty of Sciences, USJ.  Lecturer in Human Physiology, Bachelor of Science in Life and Earth Sciences, Faculty of Sciences, USJ.  Lecturer in Organization of the Animal kingdom, Bachelor of Science in Life and Earth Sciences, Faculty of Sciences, USJ.  Lecturer in Molecular Endocrinology, Master in Functional Genomics and Proteomics, Faculty of Sciences, USJ-  Coordinator of the Physiology syllabus, Faculty of Sciences, USJ. ORIGINAL RESEARCH PAPERS 1. Maalouly G, Hajal J, Noujeim C, Choueiry M, Nassereddine H, Smayra V, Saliba Y, Fares N. New insights in gut-liver axis in wild-type murine imiquimod-induced lupus. Lupus. 2021 Feb 17:-. doi: 10.1177/-. Epub ahead of print. PMID:-. Habr B*, Saliba Y*, Hajal J, Smayra V, Riachy M, Fares N. Hydrocortisone mitigates ICU-AW by fine-tuning of muscle atrophic and hypertrophic signaling pathways in a sepsis model with limb immobilization. Life Sci. 2020 Nov 15;261:118366. doi: 10.1016/j.lfs-. Epub 2020 Aug 29. PMID:-. (*co-authors) 3. Achkar A, Saliba Y, Fares N. Differential Gender-Dependent Patterns of Cardiac Fibrosis and Fibroblast Phenotypes in Aging Mice. Oxid Med Cell Longev. 2020 May 23;2020:-. doi: 10.1155/2020/-. PMID:-; PMCID: PMC-. 4. Maalouly G, Hajal J*, Saliba Y*, Rached G*, Layoun H*, Smayra V, Sleilaty G, Irani C, Fares N. Beneficial role of simvastatin in experimental autoimmune myositis. Int Immunopharmacol. 2020 Feb;79:106051. doi: 10.1016/j.intimp-. Epub 2019 Dec 18. PMID:-. (*coauthors) 5. Hajal J, Joubran N, Sleilaty G, Chacra D, Saliba Y, Assaad S, Chelala D, Fares N. Intradialytic hypotension: beyond hemodynamics. Physiol Res. 2019 Oct 25;68(5):793-805. doi:-/physiolres.934080. Epub 2019 Aug 19. PMID:-. Amara J, Saliba Y, Hajal J, Smayra V, Bakhos JJ, Sayegh R, Fares N. Circadian Rhythm Disruption Aggravates DSS-Induced Colitis in Mice with Fecal Calprotectin as a Marker of Colitis Severity. Dig Dis Sci. 2019 Nov;64(11):-. doi: 10.1007/s-. Epub 2019 May 21. PMID:-. Saliba Y, Farès N. Isolation, Purification, and Culture of Mouse Pancreatic Islets of Langerhans. Methods Mol Biol. 2019;1940:255-265. doi: 10.1007/-_18. PMID:-. Chacar S, Hajal J*, Saliba Y*, Bois P, Louka N, Maroun RG, Faivre JF, Fares N. Long-term intake of phenolic compounds attenuates age-related cardiac remodeling. Aging Cell. 2019 Apr;18(2):e12894. doi: 10.1111/acel.12894. Epub 2019 Jan 24. PMID:-; PMCID: PMC-. (*co-authors). 9. Rouhana S, Farah C, Roy J, Finan A, Rodrigues de Araujo G, Bideaux P, Scheuermann V, Saliba Y, Reboul C, Cazorla O, Aimond F, Richard S, Thireau J, Fares N. Early calcium handling imbalance in pressure overload-induced heart failure with nearly normal left ventricular ejection fraction. Biochim Biophys Acta Mol Basis Dis. 2019 Jan;1865(1):230-242. doi: 10.1016/j.bbadis-. Epub 2018 Aug 4. PMID:-. Beaini S*, Saliba Y*, Hajal J, Smayra V, Bakhos JJ, Joubran N, Chelala D, Fares N. VEGF-C attenuates renal damage in salt-sensitive hypertension. J Cell Physiol. 2019 Jun;234(6):-. doi: 10.1002/jcp.27648. Epub 2018 Oct 30. PMID:-. (*co-authors). 11. Saliba Y, Jebara V, Hajal J, Maroun R, Chacar S, Smayra V, Abramowitz J, Birnbaumer L, Farès N. Transient Receptor Potential Canonical 3 and Nuclear Factor of Activated T Cells C3 Signaling Pathway Critically Regulates Myocardial Fibrosis. Antioxid Redox Signal. 2019 Jun 1;30(16):-. doi: 10.1089/ars-. Epub 2018 Nov 29. PMID:-; PMCID: PMC-. Nakhl S, Sleilaty G, El Samad S, Saliba Y, Chahine R, Farès N. Association between vitamin D deficiency and lipid and non-lipid markers of cardiovascular diseases in the middle east region. Eur J Clin Nutr. 2019 Jun;73(6):850-858. doi: 10.1038/s-. Epub 2018 Aug 10. PMID:-. Hajal J, Saliba Y, Joubran N, Sleilaty G, Chacra D, Assaad S, Chelala D, and Farès N. New Insights into the Pathogenesis of Intradialytic Hypertension. The Open Urology & Nephrology Journal. 2018, 11, 87-99. doi: 10.2174/-X-. Bou Khalil R, Smayra V, Saliba Y, Hajal J, Bakhos JJ, Souaiby L, Richa S, Tamraz J, Farès N. Mifepristone reduces hypothalamo-pituitary-adrenal axis activation and restores weight loss in rats subjected to dietary restriction and methylphenidate administration. Neurosci Res. 2018 Oct;135:4653. doi: 10.1016/j.neures-. Epub 2017 Dec 28. PMID:-. 15. Chacar S, Itani T, Hajal J, Saliba Y, Louka N, Faivre JF, Maroun R, Fares N. The Impact of LongTerm Intake of Phenolic Compounds-Rich Grape Pomace on Rat Gut Microbiota. J Food Sci. 2018 Jan;83(1):246-251. doi: 10.1111/-. Epub 2017 Dec 11. PMID:-. Saliba Y, Bakhos JJ, Itani T, Farès N. An optimized protocol for purification of functional islets of Langerhans. Lab Invest. 2017 Jan;97(1):70-83. doi: 10.1038/labinvest-. Epub 2016 Nov 28. PMID:-. Bou Khalil R, Fares N, Saliba Y, Tamraz J, Richa S. L’effet de la méthylphénidate sur l’appétit et le poids [The effect of methylphenidate on appetite and weight]. Encephale. 2017 Dec;43(6):577-581. French. doi: 10.1016/j.encep-. Epub 2017 Mar 23. PMID:-. Maalouly G, Ward C, Smayra V, Saliba Y, Aftimos G, Haddad F, Farès N. Fish oil attenuates neurologic severity of antiphospholipid syndrome in a mice experimental model. Nutr Neurosci. 2017 Dec;20(10):563-570. doi: 10.1080/-X-. Epub 2016 Jul 17. PMID:-. Saliba Y, Karam R, Smayra V, Aftimos G, Abramowitz J, Birnbaumer L, Farès N. Evidence of a Role for Fibroblast Transient Receptor Potential Canonical 3 Ca2+ Channel in Renal Fibrosis. J Am Soc Nephrol. 2015 Aug;26(8):1855-76. doi: 10.1681/ASN-. Epub 2014 Dec 5. PMID:-; PMCID: PMC-. Saliba Y, Keck M, Marchand A, Atassi F, Ouillé A, Cazorla O, Trebak M, Pavoine C, Lacampagne A, Hulot JS, Farès N, Fauconnier J, Lompré AM. Emergence of Orai3 activity during cardiac hypertrophy. Cardiovasc Res. 2015 Mar 1;105(3):248-59. doi: 10.1093/cvr/cvu207. Epub 2014 Sep 11. PMID:-; PMCID: PMC-. (selected as Editor’s Choice) 21. Moubarak M, Magaud C, Saliba Y, Chatelier A, Bois P, Faivre JF, Fares N. Effects of atrial natriuretic peptide on rat ventricular fibroblasts during differentiation into myofibroblasts. Physiol Res. 2015;64(4):495-503. doi:-/physiolres.932839. Epub 2014 Dec 3. PMID:-. Hajje G*, Saliba Y*, Itani T, Moubarak M, Aftimos G, Farès N. Hypothyroidism and its rapid correction alter cardiac remodeling. PLoS One. 2014 Oct 15;9(10):e109753. doi: 10.1371/journal.pone-. PMID:-; PMCID: PMC-. (*co-authors). 23. Lompre AM, Benard L, Saliba Y, Aubart F, Fauconnier J, Hulot JS. STIM1 and Orai in cardiac hypertrophy and vascular proliferative diseases. Front Biosci (Schol Ed). 2013 Jan 1;5:766-73. doi: 10.2741/s406. PMID:-. Saliba Y, Mougenot N, Jacquet A, Atassi F, Hatem S, Farès N, Lompré AM. A new method of ultrasonic nonviral gene delivery to the adult myocardium. J Mol Cell Cardiol. 2012 Dec;53(6):8018. doi: 10.1016/j.yjmcc-. Epub 2012 Aug 3. PMID:-. Marchand A, Abi-Gerges A, Saliba Y, Merlet E, Lompré AM. Calcium signaling in vascular smooth muscle cells: from physiology to pathology. Adv Exp Med Biol. 2012;740:795-810. doi: 10.1007/-_35. PMID:-. Moubarak M, Jabbour H, Smayra V, Chouery E, Saliba Y, Jebara V, Fares N. Cardiorenal syndrome in hypertensive rats: microalbuminuria, inflammation and ventricular hypertrophy. Physiol Res. 2012;61(1):13-24. doi:-/physiolres.932146. Epub 2011 Dec 20. PMID:-. 27. Hulot JS, Fauconnier J, Ramanujam D, Chaanine A, Aubart F, Sassi Y, Merkle S, Cazorla O, Ouillé A, Dupuis M, Hadri L, Jeong D, Mühlstedt S, Schmitt J, Braun A, Bénard L, Saliba Y, Laggerbauer B, Nieswandt B, Lacampagne A, Hajjar RJ, Lompré AM, Engelhardt S. Critical role for stromal interaction molecule 1 in cardiac hypertrophy. Circulation. 2011 Aug 16;124(7):796-805. doi: 10.1161/CIRCULATIONAHA-. Epub 2011 Aug 1. PMID:-; PMCID: PMC-. Saliba Y, Chouery E, Mégarbané A, Jabbour H, Farès N. Microalbuminuria versus brain natriuretic peptide in cardiac hypertrophy of hypertensive rats. Physiol Res. 2010;59(6):871-80. Epub 2010 Jun 9. PMID:-. Fares N, Nader L, Saliba Y, Aftimos G, Gebara V. B-type natriuretic peptide modulates the action potential and the L-type calcium current in adult rat heart muscle cells. J Med Liban. 2010 OctDec;58(4):222-7. PMID:-. PUBLISHED ABSTRACTS 1. Nakhl S, Saliba Y, Chahine R, Birnbaumer L, Groschner K, Farès N. Non-genomic effects of calcitriol (1,25-dihydroxyvitamin D3) on transient receptor potential canonical 3 channels (TRPC3) in cardiac ventricular fibroblasts. Acta Physiologica. 2019, Volume 227, Issue S718. 2. Bakhos JJ, Saliba Y, Hajal J, Farès, N. Effects of wild-type and mutant forms of atrial natriuretic peptide on cardiac fibrosis in type-2 diabetes. Acta Physiologica. 2019, Volume 227, Issue S718. 3. Saliba Y, Jebara V, Hajal J, Maroun R, Chacar S, Smayra V, Abramowitz J, Birnbaumer L, Farès N. TRPC-NFAT signaling inhibition mediates the cardiac anti-fibrotic effect of polyphenols. Acta Physiologica. 2017, Volume 221, Issue S713. 4. Beaini S, Saliba Y, Hajal J, Smayra V, Bakhos JJ, Chelala D, Farès N. Salt-sensitive hypertension: role of vascular endothelial growth factor C and lymphangiogenesis. Acta Physiologica. 2017, Volume 221, Issue S713. 5. Saliba Y, Bakhos JJ, Farès N. A fast and reliable protocol for isolating functional rodent islets. Acta Physiologica. 2016, 217 (suppl. 708), 3-158. 6. Hajal J, Saliba Y, Chacra D, Chelala D, Farès N. Evaluation of NO homeostasis actors and VEGFC in intradialytic hypertension. Acta Physiologica. 2016, 217 (suppl. 708), 3-158. 7. Amara J, Smayra V, Saliba Y, Hajale J, Beaini C, Bakhos J, Sayegh R, Farès N. Effect of circadian disruption on inflammatory bowel disease activity: an experimental study on a DSS-induced mice colitis model. United European Gastroenterology Journal. 2016, 4(5S) A157–A720. 8. Saliba Y, Keck M, Marchand A, Atassi F, Ouillé A, Cazorla O, Trebak M, Pavoine C, Lacampagne A, Hulot JS, Farès N, Fauconnier J, Lompré AM. Emergence of Orai3 activity during cardiac hypertrophy. Acta Physiologica. 2015, 215 (S705), i-137. 9. Hulot JS, Saliba Y, Fauconnier J, Keck M, Bénard L, Marchand A, Farès N, Lompré AM. Stim/orai Channel Complex in normal And Hypertrophied Adult Cardiac Myocytes. Circulation Research. 2013, Aug; 113: A198, Issue Suppl 1. 10. Saliba Y, Chouery E, Farès N. Microalbuminuria versus Brain Natriuretic Peptide in Cardiac Hypertrophy of Hypertensive Rats. Archives of cardiovascular diseases. 2010 (2).p. 59. GRANTS, AWARDS AND FELLOWSHIPS 2019 Best poster prize - Joint Meeting of the Federation of European Physiological Societies and the Italian Physiological Society, Bologna, Italy, September- Best Oral presentation - International Society of Antioxidants in Nutrition and Health (ISANH), May 3-4, Beirut, Lebanon. 2016 Best poster prize - 25th Anniversary of the FEPS, 168th Anniversary of French Physiological Society, Paris, France, June 29th - July 1st. 2015 Travel award - Federation of European Physiological Societies and the Baltic Physiological Societies congress in Kaunas, Lithuania, August- Grant from Agence Nationale de la Recherche (ANR-10-BLAN-1102) for the project «Cardiosoc»: Dynamics of STIM/Orai/TRPC interaction in the normal, hypertrophied and failing cardiac myocyte - Inserm UMRS 956 Institut National de la Santé et de la Recherche Médicale – Délégation Paris VI et Inserm U637 Institut National de la Santé et de la Recherche Médicale – ADR Languedoc-Roussillon – ADR 8. 2010 Eiffel Excellence Scholarship - French Ministry of European and Foreign Affairs. Selection between 1400 PhD candidates. 2010 CÈDRE fellowship within the framework of collaboration between USJ and Pierre et Marie Curie University during my PhD project. 2007 Excellence and Honor awards as Major de Promotion - Lebanese University, Faculty of Science 2. ORAL COMMUNICATIONS AND POSTERS IN CONGRESSES AND SYMPOSIA 1. Amara J, Itani J, Saliba Y, Sarkis D, Farès N. Réduction de la diversité du microbiote fécal chez des souris soumises à une inversion du rythme circadien dans le cadre d'une colite DSS-induite. Journées Francophones d'Hépato-gastroentérologie et d'Oncologie Digestive. Poster. Prévu à Paris, France, 25-28 Juin 2020. 2. Bakhos JJ, Saliba Y, Hajal J, Farès, N. Effects of wild-type and mutant forms of atrial natriuretic peptide on cardiac fibrosis in type-2 diabetes. Joint meeting of the Federation of European Physiological Societies and the Italian Physiological Society. Poster. Bologne, Italie, 10-13 Septembre 2019. 3. Nakhl S, Saliba Y, Chahine R, Birnbaumer L, Groschner K, Farès N. Non-genomic effects of calcitriol (1,25-dihydroxyvitamin D3) on transient receptor potential canonical 3 channels (TRPC3) in cardiac ventricular fibroblasts. Joint meeting of the Federation of European Physiological Societies and the Italian Physiological Society. Poster. Bologne, Italie, 10-13 Septembre 2019. 4. Nakhl S, Saliba Y, Chahine R, Farès N. Mise en évidence et caractérisation d'un nouvel effet nongénomique du calcitriol (1,25-dihydroxyvitamin D3) sur les canaux TRPC3 dans les fibroblastes cardiaques ventriculaires. Journée de la Recherche, USJ. Communication orale. Beirut, mai 2019. (Prix de la meilleure communication orale). 5. Rached G, Maddah D, Saliba Y, Hajal J, Bakhos JJ, Farès N. Transient receptor potential canonical 3 (TRPC3), a new candidate implicated in insulin secretion and glycemic control. European Calcium Society Congress. Communication orale. Hamburg, Allemagne, 9-13 Septembre 2018. 6. Saliba Y, Jebara V, Hajal J, Maroun R, Chacar S, Smayra V, Abramowitz J, Birnbaumer L, Farès N. TRPC3/NFATc3 signaling pathway critically regulates myocardial fibrosis. European Calcium Society Congress. Poster. Hamburg, Allemagne, 9-13 Septembre 2018. 7. Saliba Y, Jebara V, Hajal J, Maroun R, Chacar S, Smayra V, Abramowitz J, Birnbaumer L, Farès N. TRPC-NFAT signaling inhibition mediates the cardiac anti-fibrotic effect of polyphenols. Joint Meeting of the Federation of European Physiological Societies and the Austrian Physiological Society. Communication orale. Vienne, Autriche, 13-15 Septembre 2017. 8. Beaini S, Saliba Y, Hajal J, Smayra V, Bakhos JJ, Chelala D, Farès N. Salt-sensitive hypertension: role of vascular endothelial growth factor C and lymphangiogenesis. Joint Meeting of the Federation of European Physiological Societies and the Austrian Physiological Society. Communication orale. Vienne, Autriche, 13-15 Septembre 2017. 9. Saliba Y, Hajal J, Chacar S, Smayra V, Maroun R, Farès N. Effect of phenolic compounds on the cardiac fibroblast phenotype: in vitro experimental study in rats. 18th ISANH Middle East World Congress. Communication orale. Beirut, 3-4 Mai 2017. 10. Bou Khalil R, Saliba Y, Hajal J, Richa S, Tamraz J, Farès N. Efficacité de la mifepristone dans le traitement d’un modèle d’anorexie mentale chez les rats. 15e Congrès de l’Encéphale. Communication orale. Paris, France, 18-20 janvier 2017. 11. Nakhl S, Sleilaty G, El Samad S, Saliba Y, Chahine R, Farès N. Insuffisance en vitamine D et maladies cardiovasculaires. 22nd International Scientific Conference of LAAS “The Social Avenues of Research”. Communication orale. Beirut, avril 2016. 12. Amara J, Smayra V, Saliba Y, Hajale J, Beaini C, Bakhos J, Sayegh R, Farès N. Effect of circadian disruption on inflammatory bowel disease activity: an experimental study on a DSS-induced mice colitis model. Journées Francophones d'Hépato-gastroentérologie et d'Oncologie Digestive. Poster. Paris, France, 17-20 Mars, 2016. 13. Amara J, Smayra V, Saliba Y, Hajale J, Beaini C, Bakhos J, Sayegh R, Farès N. Effect of circadian disruption on inflammatory bowel disease activity: an experimental study on a DSS-induced mice colitis model. Poster. Congré de Rhumatologie, Beirut 2016. 14. Saliba Y, Bakhos JJ, Farès N. A fast and reliable protocol for isolating functional rodent islets. Joint Meeting of the Federation of European Physiological Societies and the French Physiological Society. Poster. Paris, France, 29 Juin-1 Juillet 2016. 15. Hajal J, Saliba Y, Chacra D, Chelala D, Farès N. Evaluation of NO homeostasis actors and VEGFC in intradialytic hypertension. Joint Meeting of the Federation of European Physiological Societies and the French Physiological Society. Poster. Paris, France, 29 Juin-1 Juillet 2016. 16. Chacar S, Saliba Y, Tarhini M, Faivre JF, Maroun R, Farès N. Grape pomace extracts as antioxidants and antiradicals compounds in an in vivo rat aging model. 15th ISANH. Poster. Dubai, UAE, 12-14 Avril 2016. (Prix du meilleur poster). 17. Rouhana S, Farah C, Bideaux P, Reboul C, Saliba Y, Farès N, Richard S. Developpment of a model of diastolic heart failure with preserved ejection fraction in the rat: Functional validation in vivo. EMC. Poster. Montpellier, France, 2-6 septembre 2016. 18. Hajal J, Saliba Y, Chacra D, Chelala D, Farès N. Evaluation of NO homeostasis actors and VEGFC in intradialytic hypertension. Journées de la recherche, USJ. Poster. Beirut, Liban. septembre 2016. 19. Saliba S, Bakhos JJ, Farès N. A fast and reliable protocol for isolating functional rodent islets. Journées de la recherche, USJ. Poster. Beirut, Liban, septembre 2016. 20. Chacar S, Saliba Y, Tarhini M, Faivre JF, Maroun R, Farès N. Grape pomace extracts as antioxidants and antiradicals compounds in an in vivo rat aging model. Journées de la recherche, USJ. Poster. Beirut, Liban, septembre 2016. 21. Amara J, Smayra V, Saliba Y, Hajal J, Bakhos JJ, Beaini C, Sayegh R, Farès N. Effect of circadian disruption on inflammatory bowel diseases activity: An experimental study on a DSS-induced mice colitis model. United European Gastroenterolgy week. Poster. Vienne, Autriche, 15-19 octobre 2016. 22. Amara J, Smayra V, Saliba Y, Hajal J, Bakhos JJ, Beaini C, Sayegh R, Farès N. Effect of circadian disruption on inflammatory bowel diseases activity: An experimental study on a DSS-induced mice colitis model. Congrès de Rhumatologie. Poster. Beirut, Liban, 2016. 23. Farah C, Rouhana S, Reboul C, Bideaux P, Saliba Y, Farès N, Richard S. Développement d’un modèle d’insuffisance cardiaque à fraction d’éjection préservée chez le rat : évaluation fonctionnelle in vivo. Printemps de la cardiologie, GRRC. Poster. Dijon, France, 7-8 avril 2016. 24. Moubarak M, Magaud C, Saliba Y, Chatelier A, Bois P, Faivre JF, Farès N. Effects of atrial natriuretic peptide on rat ventricular fibroblasts during differentiation into myofibroblasts. Printemps de la cardiologie, GRRC. Poster. Toulouse, France, 2-3 avril 2015. 25. Hajal J, Saliba Y, Chacra D, Assaad C, Chelala D, Farès N. Nouvelles pistes dans les mécanismes physiopathologiques de l’hypertension artérielle perdialytique. JFL3. Communication orale. HadathUL, Liban. 29-30 octobre 2015. 26. Saliba Y, Karam R, Smayra V, Aftimos G, Abramowitz J, Birnbaumer L, Farès N. Evidence of a Role for Fibroblast Transient Receptor Potential Canonical 3 Ca2+ Channel in Renal Fibrosis. LAAS21-USJ. Poster. Beirut, Liban, 15-17 Avril 2015. 27. Moubarak M, Magaud C, Saliba Y, Chatelier A, Bois P, Faivre JF, Farès N. Effects of atrial natriuretic peptide on rat ventricular fibroblasts during differentiation into myofibroblasts. LAAS21USJ. Poster. Beirut, Liban, 15-17 Avril 2015. 28. Saliba Y, Keck M, Marchand A, Atassi F, Ouillé A, Cazorla O, Trebak M, Pavoine C, Lacampagne A, Hulot JS, Farès N, Fauconnier J, Lompré AM. Emergence of Orai3 activity during cardiac hypertrophy. Joint Meeting of the FEPS and the Baltic Physiological Socities. Communication orale. Kaunas, Lithuanie, 26-29 Août 2015. 29. Saliba Y, Fauconnier J, Keck M, Bénard L, Marchand A, Hulot JS, Farès N, Lompré AM. STIM/Orai channel complex in normal and hypertrophied adult cardiac myocytes. International Society for Heart Research XXI World Congress. Poster. San Diego, California, USA, 30 Juin-4 Juillet 2013. 30. Saliba Y, Fauconnier J, Keck M, Bénard L, Marchand A, Hulot JS, Farès N, Lompré AM. STIM/Orai channel complex in normal and hypertrophied adult cardiac myocytes. Basic Cardiovascular sciences. Poster. Paris Las Vegas, USA, 22-25 Juillet 2013. 31. Saliba Y, Karam R, Smayra V, Aftimos G, Farès N. TRPC3 inhibition prevents renal fibrosis induced by unilateral ureteral obstruction. International Union for Physiological Sciences. Poster. Birmingham, UK, 21-26 Juillet 2013. 32. Saliba Y, Keck M, Marchand A, Atassi F, Ouillé A, Mougenot N, Jacquet A, Lacampagne A, Hulot JS, Farès N, Fauconnier J, Lompré AM. Defining STIM1 partners in cardiac hypertrophy. Gordon Research Conferences, Calcium Signaling. Poster. Lucca, Italy, 16-21 Juin 2013. 33. Saliba Y, Fauconnier J, Bénard L, Marchand A, Farès N, Hulot JS, Lompré AM. Dynamics of the STIM/Orai/TRPC channel complex in normal and hypertrophied adult cardiac myocytes. European Calcium Society 12th Meeting. Communication orale. Toulouse, France, 9-12 Septembre 2012. 34. Saliba Y, Fauconnier J, Lacampagne A, Farès N, Lompré AM. Dynamics of the STIM/ORAIs/TRPCs channel complex in normal and hypertrophied rat cardiac myocytes. Printemps de la Cardiologie, Recherche Fondamentale et Clinique. Poster. Bordeaux, France, 12-13 Avril 2012. 35. Saliba Y, Mougenot N, Jacquet A, Atassi F, Hatem S, Farès N, Lompré AM. A new efficient method of ultrasonic non viral gene delivery to the rat myocardium. Congrès de Physiologie, Pharmacologie et Thérapeutique (P2T). Poster. Dijon, France, 4-6 Avril 2012. 36. Saliba Y, Fauconnier J, Lacampagne A, Farès N, Lompré AM. Dynamics of the STIM/ORAIs/TRPCs channel complex in normal and hypertrophied rat cardiac myocytes. 1st International Meeting on Ion Channel Signaling Mechanisms, from basic science to clinical application. Poster. Marrakech, Maroc, 31 Octobre-4 Novembre 2011. 37. Hulot JS, Fauconnier J, Ramanujam D, Chaanine A, Aubart F, Sassi Y, Merkle S, Cazorla O, Ouillé A, Dupuis M, Hadri L, Jeong D, Mühlstedt S, Schmitt J, Braun A, Bénard L, Saliba Y, Laggerbauer B, Nieswandt B, Lacampagne A, Hajjar RJ, Lompré AM, Engelhardt S. Critical role for stromal interaction molecule 1 in cardiac hypertrophy. 1st International Meeting on Ion Channel Signaling Mechanisms, from basic science to clinical application. Communication orale. Marrakech, Maroc, 31 Octobre-4 Novembre 2011. 38. Hulot JS, Fauconnier J, Ramanujam D, Chaanine A, Aubart F, Sassi Y, Merkle S, Cazorla O, Ouillé A, Dupuis M, Hadri L, Jeong D, Mühlstedt S, Schmitt J, Braun A, Bénard L, Saliba Y, Laggerbauer B, Nieswandt B, Lacampagne A, Hajjar RJ, Lompré AM, Engelhardt S. Critical role for stromal interaction molecule 1 in cardiac hypertrophy. European Society of Cardiology Congress. Communication orale. Paris, France, 27-31 Août 2011. 39. Saliba Y, Mougenot N, Jacquet A, Atassi F, Farès N, Lompré AM. Dynamique de l’interaction du complexe canalaire STIM1/ORAIs/TRPCs» dans les cardiomyocytes normaux et hypertrophiés du rat adulte. Journée Scientifique Annuelle de l’Institut Fédératif de Recherche Cœur Muscle Vaisseaux. Poster. Paris, France, 17 Mars 2011. 40. Saliba Y, Chouery E, Jabbour H, Mégharbané A, Moubarak M, Farès N. Microalbuminuria versus Brain Natriuretic Peptide in Cardiac Hypertrophy of Hypertensive Rats. Printemps de la Cardiologie, Recherche Fondamentale et Clinique. Poster. Nantes, France, 15-17 Avril 2010. 41. Merlet E, Saliba Y, Lompré AM, Marchand A. Rôle des microARNs dans le remodelage lié au calcium du système cardio-vasculaire. Printemps de la Cardiologie, Recherche Fondamentale et Clinique. Poster. Nantes, France, 15-17 Avril 2010. 42. Saliba Y, Mougenot N, Jacquet A, Atassi F, Farès N, Lompré AM. Dynamique de l’interaction du complexe canalaire STIM1/ORAIs/TRPCs» dans les cardiomyocytes normaux et hypertrophiés du rat adulte. Journée Scientifique Annuelle de l’Institut Fédératif de Recherche Cœur Muscle Vaisseaux. Poster. Paris, France, 15 Avril 2010. 43. Saliba Y, Mougenot N, Jacquet A, Atassi F, Farès N, Lompré AM. Dynamique de l’interaction du complexe canalaire STIM1/ORAIs/TRPCs» dans les cardiomyocytes normaux et hypertrophiés du rat adulte. Journée Scientifique de l’Ecole Doctorale Physiologie et Physiopathologie – UPMC. Poster. Paris, France, 3-4 Juin 2010. COMPLEMENTARY FORMATION March 2014 Level 1 Medical Pedagogy training, Faculty of Medicine, USJ, Beirut. January, February 2010 Training in Confocal Microscopy at the Brain and Spinal Cord Institute, Pitié Salpêtrière, UPMC, Paris. November 2009 UPMC, Paris. Formation in Scientific Methodology at the Institute of Doctoral Formation – October 2009 Formation in Flow Cytometry level 1 (theory and practice) at the Cordeliers Research Center, Paris. August 2006 Cytogenetics internship at the Medical Genetics Laboratory – Saint Joseph University USJ, under the supervision of Pr. André MÉGARBANÉ. June 2006 Cytogenetics and Molecular Biology internship at The Thalassemia Center “Chronic Care” – Baabda, under the supervision of Pr. Chantal FARRA. TECHNICAL SKILLS 1. Physiology - Live-cell imaging: Intracellular calcium (probes indo-1, fura-2 and fluo-4) on a variety of primary cells and cardiomyocyte contractility measurements with Ionoptix system. Measuring intracellular cGMP by a fluorescent probe expressed in cells by a BACMAM. - Electrophysiology: patch clamp (general view). - Echocardiography on small rodents - Microsurgery: cardiac hypertrophy induction in the rat by abdominal aortic constriction and renal artery ligation (2K-1C, 1K-1C). Ureteral obstruction model in the rat. Osmotic pumps implantation. - Animal models: cardiac hypertrophy, heart failure with preserved and reduced ejection fraction, ischemia reperfusion, diabetes type 1 and 2, aging, anorexia, circadian rhythm inversion, colitis, various models of hypertension, myositis, various models of sepsis. - Isolation, dissociation and primary cell cultures of smooth muscle cells, cardiac fibroblasts and cardiomyocytes (rat and mouse), renal fibroblasts, islets of Langerhans, osteoblasts, hepatocytes, and others. - Isolation and culture of cardiac fibroblasts from human biopsies. - Isolation and culture of blood cells. - Blood pressure measurement via tail-cuff method in rats and mice. 2. Cellular and Molecular Biology/Biochemistry - In vivo cardiac gene transfer (siRNA, plasmid, adenovirus, AAV and lentivirus) in rats and mice. - Amplification and production of lentiviruses, adenoviruses and adeno-associated viruses (AAV) for over- or down-regulating gene expression by RNA interference. - Immunofluorescence and data acquisition by confocal and epifluorescence microscopy. - Different technics of cell culture transfection, bacterial transformation and plasmid DNA amplification. - Cell proliferation and cytotoxicity tests. - RNA extraction, RT-PCR, quantitative PCR, gene sequencing (global view), electrophoresis. - Lymphocyte culture, chromosomes classification and caryotype interpretation. - Extraction, quantification and immunological dosage of proteins by ELISA. - Various cellular enzymes activity assays. - Western Blot, Co-Immunoprecipitation. - Histology and immuno-histochemistry. LINGUISTIC SKILLS Arabic Mother tongue. French Excellent level. Advanced level according to the Common European Framework of Reference for Languages English Excellent level. Certificate of Competency in English Computer skills: Image J, Word, Excel, Powerpoint, Access and Internet. Bibliography tools ‘‘Pubmed, NCBI, UCSC genome browser, Embl-Ebi’’. Oligonucleotides design for RNA interference and PCR. REFERENCES - Nassim FARÈS, PhD. Director of the Physiology and Pathophysiology Research Laboratory Faculty of Medicine, Saint Joseph University. - Anne-Marie LOMPRÉ, PhD. Director of the team ‘‘Cellular signaling and new therapeutic approaches of cardiovascular remodeling’’, Inserm U 956 ‘‘Physiology, genetics and cardiovascular pharmacology’’ (Recently known as: Inserm UMRS 1166, ICAN – Institute of CardioMetabolism and Nutrition), Faculty of Medicine, Pitié Salpêtrière, Pierre and Marie Curie University, Sorbonne University. - Stéphane HATEM, MD, PhD. Director of the Inserm U 956 ‘‘Physiology, genetics and cardiovascular pharmacology’’ (Recently known as: Inserm UMRS 1166, ICAN – Institute of CardioMetabolism and Nutrition), Faculty of Medecine, Pitié Salpêtrière, Pierre and Marie Curie University, Sorbonne University.