William J. Murphy

CURRICULUM VITAE William J. Murphy, Ph.D. Date of Preparation: 02/18/19 PERSONAL DATA: Birth date: Birthplace: Citizenship Status: EDUCATION: Ph.D. 1984 B.S. 1978 1955 Minneapolis, MN USA Department of Biochemistry, University of Iowa, Iowa City, IA University of Minnesota, Minneapolis–St. Paul, MN (Major: Biochemistry) PROFESSIONAL EXPERIENCE: 2010–present Self-employed (Business website consultant and developer, MH Home Rentals, LLC; TaxArrest.com; House Call Docs, CS, LLC, Murphy Appliance Repair, LLC- Adjunct Assistant Professor, Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS- Research Assistant Professor, Wilkinson Laboratory of the Kansas Cancer Institute and Departments of Pathology & Laboratory Medicine and Internal Medicine, University of Kansas Medical Center, Kansas City, KS- Assistant Professor, Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS- Postdoctoral Research Scientist. Advisor, Dr. Nina Agabian, Department of Biochemistry, University of California Berkeley, Berkeley, CA and Department of Pharmacy, University of California San Francisco–Laurel Heights, San Francisco, CA- Graduate Assistant. Advisor, Dr. John E. Donelson, Department of Biochemistry, University of Iowa, Iowa City, IA- Undergraduate Research Assistant. Advisor, Dr. Lavell M. Henderson, Department of Biochemistry, University of Minnesota, St. Paul, MN TEACHING EXPERIENCE: Course Responsibilities, University of Kansas Medical Center:- Medical Microbiology: Lectures on parasites of medical relevance- Advanced Microbial Molecular Genetics–Eukaryotes: Topics in eukaryotic molecular biology, transcription, RNA splicing, transposable elements- Graduate Student Seminar Series: Guidance of graduate students in the presentation of seminars on journal articles- Medical Microbiology: Lectures on parasites of medical relevance. PUBLICATIONS: 1. Donelson, J. E., Murphy, W. J., Brentano, S. T., Rice-Ficht, A. C. and Cain, G. D. (1983) Comparison of the expression-linked extra copy (ELC) and basic copy (BC) genes of a trypanosome surface antigen. J. of Cell. Biochem. 23, 1-12. 1 2. Murphy, W. J., Brentano, S. T., Rice-Ficht, A. C., Dorfman, D. M. and Donelson, J. E. (1983) DNA rearrangements of the variable surface antigen genes of the trypanosomes. J. Protozool. 31, 65-73. 3. Parslow, T. G., Blair, D. L., Murphy, W. J., and Granner, D. K. (1984) Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence. Proc. Natl. Acad. Sci. USA 81,-. Murphy, W. J., Watkins, K. P., and Agabian, N. (1986) Identification of a novel Y branch structure as an intermediate in trypanosome mRNA processing: evidence for trans splicing. Cell 47,-. Mottram, J. C., Murphy, W. J., and Agabian, N. (1989) A transcriptional analysis of the Trypanosoma brucei hsp83 gene cluster. Mol. Biochem. Parasitol. 37,- Lorsbach, R. B., Murphy, W. J., Lowenstein, C. J., Snyder, S. H. and Russell, S. W. (1992) Expression of the nitric oxide synthase gene in mouse macrophages activated for tumor cell killing: molecular basis for the synergy between IFN-gamma and lipopolysaccharide. J. Biol. Chem. 268,-. Lowenstein, C. J., Alley, E. W., Raval, P., Snowman, A. M., Snyder, S. H., Russell, S. W., and Murphy, W. J. (1993) Macrophage nitric oxide synthase gene: Two upstream regions mediate induction by interferon γ and lipopolysaccharide. Proc. Natl. Acad. Sci. USA 90,-. Henderson, S. A., Lee, P. H., Aeberhard, E. E., Adams, J. W., Ignarro, L. J., Murphy, W. J., and Sherman, M. P. (1994) Nitric Oxide reduces early growth response-1 gene expression in rat lung macrophages treated with interferon-γ and lipopolysaccharide. J. Biol. Chem. 269,-. Raval, P., Obici, S., Russell, S. W. and Murphy, W. J. (1995) Characterization of the 5' flanking region and gene encoding the mouse interferon-gamma receptor. Gene 154,-. Alley, E. W., Murphy, W. J. and Russell, S. W. (1995) A classical enhancer element responsive to both lipopolysaccharide and interferon-γ augments induction of the iNOS gene in mouse macrophages. Gene 158,-. Zhang, X., Laubach, V. E., Alley, E. W., Edwards, K. A., Sherman, P. A., Russell, S. W., and Murphy, W. J. (1996) Transcriptional basis for hyporesponsiveness of the human inducible nitric oxide synthase gene to lipopolysaccharide/interferon-γ. J. Leukoc. Biol. 59,-. Miller, L., Alley, E. W., Murphy, W. J., Russell, S. W., and Hunt, J. S. (1996) Progesterone inhibits inducible nitric oxide synthase gene expression and nitric oxide production in murine macrophages. J. Leukoc. Biol. 59,-. Murphy, W. J., Muroi, M., Zhang, X., Suzuki, T., and Russell, S. W. (1996) Both basal and enhancer κB elements are required for full induction of the mouse inducible nitric oxide synthase gene. J. Endotoxin Res. 3,-. Gao, J., Morrison, D. C., Parmely, T. J., Russell, S. W., and Murphy, W. J. (1997) An interferon-γ activated site (GAS) is necessary for full expression of the mouse iNOS gene in response to interferon-γ and LPS. J. Biol. Chem., 272,-. Laubach, V. E., Zhang, C. X., Russell, S. W., Murphy, W. J., and Sherman P. A. (1997) Analysis of expression and promoter function of the human inducible nitric oxide synthase gene in DLD-1 cells and monkey hepatocytes. Biochim. Biophys. Acta, 1351,-. Sawada, T., Falk, L. A. Rao, P., Murphy, W. J., and Pluznik, D. H. (1997) IL-6 induction of protein DNA complexes via a novel regulatory region of the inducible nitric oxide synthase gene promoter: role of octamer binding proteins. J. Immunol., 158,-. Gao, J. J., Filla, M. B., Fultz, M. J., Vogel, S. N., Russell, S. W., and Murphy, W. J. (1998) Autocrine/ paracrine IFN-αβ mediates the lipopolysaccharide-induced activation of transcription factor 2 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. Stat1α in mouse macrophages: pivotal role of Stat1α in induction of the inducible nitric oxide synthase gene. J. Immunol., 161,-. Gay, R. D., Dawson, S. J., Murphy, W. J., Russell, S. W., and Latchman, D. S. (1998) Activation of the iNOS gene promoter by Brn-3 POU family transcription factors is dependent upon the octamer motif in the promoter. Biochim. Biophys. Acta, 1443, 315-322. Crowder, C. W., Murphy, W. J., Russell, S. W., and LeBlanc, P. A. (1999) Vesicular stomatitis virus infected BALB/c3T3 cells inhibit the synthesis of nitric oxide in activated murine bone marrow culture-derived macrophages. J. Leukoc. Biol., 65, 605–613. Chu, W., Gao, J., Murphy, W. J., and Hunt, J. S. (1999) A candidate interferon-gamma activated site (GAS element) in the HLA-G promoter does not bind nuclear proteins. Hum. Immunol. 60,-. Crespo, A., Filla, M. B, Russell, S. W., and Murphy, W. J. (2000) Indirect induction of SOCS-1 in macrophages stimulated with bacterial lipopolysaccharide: partial role of autocrine/paracrine IFN-α/β. Biochem. J., 349, 99–104. Gao, J. J., Filla, M. B., Lorsbach, R. B., Pace, J. L., Crespo, A., Russell, S. W., and Murphy, W. J. (2000) Prolonged exposure of mouse macrophages to IFN-β suppresses transcription of the inducible nitric oxide synthase gene: altered availability of transcription factor Stat1α. Eur. J. Immunol. 30,- O’Brien-Ladner, A. R., Nelson, S. R., Murphy, W. J., Blumer, B. M., and Wesselius, L. J. (2000) Iron is a regulatory component of human IL-1β production: support for regional variability in the lung. Am. J. Resp. Cell. Mol. Biol. 23, 112–119 Crespo, A., Filla, M. B., and Murphy, W. J. (2002) Low responsiveness to IFN-γ, after pretreatment of mouse macrophages with LPS, develops via diverse regulatory pathways. Eur. J. Immunol. 32, 710–719. Sur, R., Heck, D. E., Mariano T. M., Jin, Y., Murphy, W. J., and Laskin, J. D. (2002) UVB light suppresses nitric oxide production by murine keratinocytes and macrophages. Biochemical Pharmacology, 64,-. Zhang YH, Murphy WJ, Russell SW, Morrison DC, Koide N, Yoshida T, Yokochi T. (2005) Serumdependent potentiation of lipopolysaccharide-induced nitric oxide production is mediated by the events after the transcription of inducible type of nitric oxide synthase. Cell Immunol. 234, 16–22. Dhillon NK, Murphy WJ, Filla MB, Crespo AJ, Latham HA, O'Brien-Ladner A. (2009) Down modulation of IFN-gamma signaling in alveolar macrophages isolated from smokers., Toxicol Appl Pharmacol., 237, 22–28. BOOK CHAPTERS: 1. Agabian, N., Perry, K. L., and Murphy, W. J. (1987) RNA joining and trypanosome gene expression. In Molecular Biology of RNA: New Perspectives, M. Inouye and B. S. Dudock, eds. (San Diego: Academic Press) pp-. Wesselius, L. J., Murphy, W. J., Russell, S. W., and Morrison, D. C. (1997) Macrophage activation: concepts and principles applicable to the lung. In Lung Macrophages and Dendritic Cells, M. F. Lipscomb and S. W. Russell, eds. (New York: Marcel Dekker, Inc.) pp. 33–86. 3. Murphy, W. J. (1999) Transcriptional control of the genes encoding nitric oxide synthase. In Cellular and Molecular Biology of Nitric Oxide, J. D. Laskin and D. L. Laskin, eds. (New York: Marcel Dekker, Inc.), pp. 1–56. 3