Shraboni Palley

SHRABONI PALLEY E-mail:- E-mail:- Phone:- C/O surajit palley, Joydeep apartment, 40/19/1 Nandan kanan road rahara Khardaha, Kolkata-700118. I have a passion in everything I do to serve, learn, grow and maximize opportunities. Summary I believe the best path to success is by doing what I do best everyday (using my Strengths); by this philosophy, there is no limit to what can be learned or accomplished.  In everything I do, I want to learn, serve and teach. My ambition is to be in a position where I can do that every day! Additionally I want to be part of an organization that is crazy enough to believe they can change the world because we can--one person, one product, one interaction, one connection at a time. My Gallup Top 5 Strengths are: Context, Learner, Maximizer, Input, and Intellection. I live by these professionally and personally. I believe knowing my strengths and the strengths of my team members builds deeper, richer connections that lead to successes greater than we can imagine. Educational qualification Master of Science in Biochemistry First class (64%) (MAY 2007) University of Pune, Department of chemistry Bachelor of Science First Class with distinction. (82.5%) (MAY 2005) University of Pune, Nowrosjee wadia college H.S.C. Specialization-Science. First class (72.2%) (MAY 2002) Central board of secondary education (CBSE Board), K.V.BEG Pune LAB EXPERIENCE and SKILLS University of Pune, Department of chemistry, Biochemistry branch Departmental Work (Project) at post graduation Title: Comparative biodegradation of azo-dyes by alkaliphiles and neutrophiles. PROJECT HISTORY: Isolation of Pseudomonas Orizihabitans and Bacillus Sphericus. Kinetic studies- Effect of pH, temperature, external carbon source, salinity and different concentration of the dye on decolorization pattern. Enzymatic studies- Activity of Cytochrome P-450, Cytochrome b-5, Azo-reductase, Superoxide dismutase Glutathione-S-Transferees in response to dyes. Spectrophotomeric studies and IR studies before and after decolorization. Reference: kulla.H.G. (1981) Microbial biodegradation of Xenobiotics Recalcitrant Compounds. Academic Press, London. Experience Executive-chemist (Instrumentation): Mitra SK Pvt. Ltd. March 2019-Till date Analysis of artificial sweeteners, nots, caffeine, uric acid, aflatoxins, Ochratoxin, dyes, vitamins and many more on HPLC. Determination of fatty acid content (FAME )and TPH by using GC-FID for oils, and many food products. Analysis of gaseous compounds like CO2, CO, methane and non-methane compounds by using GC-FID with methanizer. Sample preparation and analysis of Pesticide residue in food products, drinking water and effluent water on GC/MS/MS and LC/MS/MS. Analysis of bromated for package drinking water by using IC and anions in ACID MIST for air samples. Working standard qualification, maintenance of working standards and certified reference standards. Method validation and uncertainty calculation. Quality analyst Supervisor Sept 2017- Feb 2019 Analysis of food and agricultural samples for pesticide residues. Coordinate with supervisor in residue analysis lab. Maintain technical records related to testing. Calibration and maintenance of LC-MS-MS, HPLC. Preparation of CRMs and mother stock standards. Analysis of samples for pesticide antibiotic residues. Junior executive-chemist (Instrumentation): Mitra SK Pvt. Ltd. Dec 2015- Aug 2017 Analysis of artificial sweeteners, nots, caffeine, uric acid, aflatoxins, dyes, vitamins and many more on HPLC. Determination of fatty acid content (FAME )and TPH by using GC-FID for oils, and many food product Analysis of bromate and chloride for package drinking water by using IC. Working standard qualification, maintenance of working standards and certified reference standards. QA/QC-Executive: Gena Pharmaceuticals Pvt.Ltd May 2015- Dec 2015 Analysis of stability samples. Online monitoring of stability chambers, photo stability chambers. Analysis of Finished products, Raw materials and in process by using calibrated instruments and conventional methods. Documentation as per cGLP Adhere; follow and implement the Standard Operating Procedures (SOPs), health and safety practices/ policies. Research scientist: Edward food research and analytical centre July2013- Feb2014 Sample preparation for Pesticide residues, Sulfa drugs, Antibiotics, Chloramphenicol, Dyes. Working standard qualification, maintenance of working standards and certified reference standards. Raw data review and compilation for sample analysis. Sending reports by LIMS system. Calibrating instruments like pH meter, Autotitrator, UV, IR, Analytical balance. Research technician: GCC Biotech Pvt.Ltd  Apr 2013 to May 2013 Project description – “Study of biodiversity of rice by genotype analysis” Extraction of genomic DNA from rice leaves obtained from different regions of India Amplification of specific regions of DNA by PCR using SSR primers. Genotypic analysis of PCR product for by Bioanalyser. R & D Scientist: Privi Organics Pvt.Ltd. (Navi Mumbai) May 2010 – FEB 2012 Biocatalysis to synthesis long chain esters involving optimization studies on the basis of concentration ratios, pH of the solution, rpm studies, temperature, enzyme concentration, also reusability study of the enzyme. Downstream processing involving optimization of the column, loading volume with respect to the rate of catalysis and ensuring the purity of the product by analysis through GC-GC/MS. Analysis of enzyme stability and product stability .Extraction and purification of enzyme and their application into aroma synthesis. Microbial synthesis of natural flavor. Teaching Experience (Pune) Aug2007 – March2010 Teaching Profile: 3 years of experience as a Science Teacher Profound knowledge of English language and ability to teach the language for all sorts of students i.e. for those who have English as their first language and those who have not. Highly skilled in using the deferent course books and material for teaching chemistry and biology as well as having a great passion and love for chemistry. Ability to dramatize stories effectively and creativeness in teaching process. Ability to plan, collect material and deliver the lessons in the class. Capacity to arrange the competitions and assess the performance of the students. Ability to inspire the students for desired effects in the language teaching task. Worked as a fundraiser for CRY Simultaneously A small contribution to the society as a human being Exploring different level of personalities at different regions of the country and collect funds for the beautiful children of the country by using my interpersonal skills. Technical expertise Familiar with HPLC (UV/RI), IC, GC-FID LC-MS/Ms, GC/MS, sample preparation, extraction to avail the required molecule of interest. Familiar with Enzyme Purification and Characterization. Familiar with various isolation & analytical techniques like DNA isolation, Chromatography (paper, column, thin layer & ion exchange), Agarose Gel Electrophoresis, Polymerase Chain Reaction (PCR) etc. Familiar with Spetrophotometric techniques, laminar work, isolation and purification of enzymes under controlled condition by cold centrifuge, sonication and determination of activity under various situations. Biophysical techniques, Clinical experiments. Familiar with ISO 17025 Attended audits for NABL, APEDA etc. Areas of Interest Mass spectroscopy, chromatography. Pesticide residue, food, drug, pharmaceuticals and cosmetics. Cellular & Molecular Biology Immunology Curricular Activities Participation in poster presentation at University of Pune, Department of chemistry (biochemistry) First prize in debate competition at school level. Acted as volunteer in an art exhibition-“DARSHAN”. Computer Awareness Basic computer knowledge- MS-CIT with 89%. Familiar with Oracle and LIMS and IMS systems. Reference persons Dr.K.R.Gawai, Department of chemistry (Biochemistry), University of Pune -411007. Phone. No (M) - -, Email ID:- Dr. Kisan Kodam, Reader Department of chemistry (biochemistry), University of Pune -411007, Phone. No (M)-, Email ID-,-Personal details Date of birth : 8th October 1983. Nationality : Indian Marital status : Married Languages known : English, Hindi, and Bengali DECLARATION I hereby declare that all the details mentioned above are true to the best of my knowledge. Shraboni Palley