CURRICULUM VITAE
PALAK SHRIVASTAVA
M.Sc ( Biotech)
CSIR- NET LS 2019, GATE-2021
Dept. of biotechnology
Dr. Harisingh gour university, Sagar, MP
E- mail :-Mobile No.:-
Objectives
To get a good career opportunity for enhancing my knowledge and capabilities by working in a dynamic organization that prides itself in giving substantial responsibility to new talent. Looking forward to achieve the pinnacle in the sphere of biotechnology and clinical/medical diagnostics with the zeal to learn and utilize the best part of experience gained from of it in an efficient manner.
Research Interest:
I am interested to combine microbiology, biotechnology, nanotechnology, electroanalytical chemistry, and molecular biology approaches to address problems of biomedical significance and diagnostics.
Medical / Clinical Diagnostics (Cancer cells, DNA, RNA, and bio-markers).
Biosensors and Nanosensors based on biological phenomenon.
Microchips and Microfluidic / Integrated devices.
Medical Microbiology and Biotechnology.
Worked for one year in ICAR- National Institute of High Security Animal Diseases (NIHSAD).
The primary objective of the work was to develop an indirect ELISA assay for the detection of Crimean-Congo Haemorrhagic fever (CCHF) virus. Recombinant nucleoprotein from Crimean-Congo Haemorrhagic Fever (CCHF) virus was successfully derived from a bacterial expression system and purified for use in a novel enzyme-linked immunosorbent assay (ELISA) diagnostic test. ELISA tests were performed for detection of IgG antibodies, thus allowing efficient detection of antibodies. The major benefits of the assay also included removing any requirement for polyclonal sera, thus eliminating variation in preparations and allowing standardization between laboratories. The assay was successfully tested using a panel of positive sera supplied from samples identified as being positive. It is envisaged that this simple diagnostic ELISA for CCHF virus infection which removes the reliance on polyclonal antibody preparations, will be accessible to a wider range of laboratories enabling them to carry out routine diagnosis. This will improve the efficiency of diagnosis and subsequent management of infected animals.
Academic Qualification
------EDUCATIONAL SNAPSHOT------
Master of Science (M.Sc) in Biotechnology from DR.Harisingh Gour University in (http://www.dhsgsu.ac.in/) in 2017 with 9.36 CGPA in scale of-)
Supervisor
Dr (Ms.) Monica Sachdev
Senior Scientist
Endocrinology
CSIR-CDRI ,
BS-10/1,Sector10,Jankipuramextension,SitapurRoad,
Lucknow 226031, India
Thesis: Theraupetic effect of Chebulinic Acid On Beningn Prostatic Hyperplasia ( BPH)
Abstract:
The aim of the study was to investigate the effect of chebulinic acid on the Benign Prostatic Hyperplasia condition . The dried fruits (500 g) of Termanalia chebula were pulverized and grinded. The grinded powder was then used for isolating an active component of it, chebulinic acid. 12-week-old(3 month) male Sprague-Dawley (SD) rats (n = 28) with initial body weights of 200-220g were taken from National Animal Laboratory Center, Central drug research institute (CDRI) To assess the therapeutic effect of chebulinic acid on Benign Prostatic Hyperplasia (BPH), rat BPH model has been successfully generated by testosterone induction method. normal control group was set up,. After the pre-treatment of 28 days, the prostate was dissected out to carry histological studies in order to confirm the establishment of model. Then BPH induced rats were randomly divided into four groups, and the rats those did not receive TP treatment became the normal control group. Thus, the rats were divided into five groups with 4 animals in each group: (a) a normal control group that received ethanol with edible oil; (b) a BPH group that received TP soluble in edible oil (c) a positive control group that received finasteride (Fi) (1 mg/kg), a 5ARI which is frequently used as a treatment for BPH , with TP (5 mg/kg); and (d) a group that received Chebulinic acid (50 mg/kg) with TP (5 mg/kg).(e) a group that received Chebulinic acid (100 mg/kg) with TP (5 mg/kg), and then for assessing the effect of Chebulinic acid Prostate weight index calculation was done of all group of rats along with Histopathical examination. Treatment with Chebulinic Acid significantly suppressed the development of prostatic hyperplasia and the prostatic weight was significantly reduced by Chebulinic Acid and histological indices were affected. Histopathological examination of BPH rats revealed glandular hyperplasia of the prostate accompanied with numeral secretions, whereas the rats treated with Chebulinic Acid showed mild glandular hyperplasia. The result suggests that Chebulinic Acid might inhibit the development and progression of BPH induced by Testosterone.
In conclusion, the present study showed that administration of Chebulinic Acid to BPH rats decreased the prostate weight and restored the altered histological architecture of the rats. Hence, from a clinical point of view, Chebulinic Acid could have applications in elderly subjects with BPH.
Bachelor of Science (B.Sc) in Biotechnology, Chemistry and Zoology from, Govt. Motilal Vigyan Mahavidhalaya, Mp (http://www.mphighereducation.nic.in/) in 2003 with 83.76% (First Division)
Project: Plant Tissue Culture, Internship.
Higher Secondary (12th) from Red rose Group of higher secondary school (http://www.redroseschools.com/contact.html) M.P. Board in 2010 with 80 % (First Division)
High School (10th) from Red rose Group of higher secondary school) (http://www.redroseschools.com/contact.html) M.P Board in 2008 with 85% (First Division)
Awards:
Got Gold Medal in Master’s education. ( M.Sc)
Trainings Undergone
Conferences / Meetings/ Symposium/ Workshops attended:
Internship on Plant Tissue Culture,Tissue Blossom Pharma Biotech Institute.
Lecture workshop on “ Scope of Medical Biotechnology” by Dept. of Biotechnology, Dr. Harisingh Gour University, Sagar, MP. (http://www.dhsgsu.ac.in/)
Summer school programme on “ Molecular cell Biology and Genetic Engineering Application” by Dept. of Biotechnology, Dr. Harisingh Gour University, Sagar, MP.
One day “ PCR Woskshop ” by Blossom Pharma Biotech Institute, Bhopal, MP.
IT Wizard Curriculum ( NIIT) – Certificate Participation.
Lab Aboard Science Express – BASF – Certificate Participation.
Interschool Quiz Competition - Certificate Participation
GIAN Initiative Course on “ Liquid chromatography and Chemometric development Method”.
Six Month Dissertation work with Dr. Monica Sachdev, Senior Scientist, Reproduction Biology Lab, Central Drug Research Institute on topic “Theraupetic effect of Chebulinic Acid On Beningn Prostatic Hyperplasia ( BPH) “
Have done internship on Plant Tissue Culture in Blossom Biotechnology Institute (Bhopal).
Experience
Genomic and Plasmid DNA isolation from different biological samples analysisPCR, RT PCR, Multiplex PCR.
Cloning and sequencing.
SDS-PAGE electrophoresis
2-D page.
EITB.
Western Blotting.
2-D page.
EITB.
Western Blotting.
Silver Staining of Protein.
Competent Cell Preparation.
Bacterial Transformation.
Routine Microbiological Techniques applied in clinical laboratories.
Technical Skills:
Fundamental knowledge of Internet Browsing, concepts of C, MS Office. Worked on different Bioinformatics Software’s : ,BLAST , FASTA, Phylogenetic analysis with Phylip.
Achievements:
Science Fair and National Science Day celebration – 2016 , Dr.Harisingh Gour University, Sagar, MP. (http://www.dhsgsu.ac.in/)
Second Prize ( Poster Presentation)
Second Prize ( essay competition)
Third Prize ( Model Presentation)
Intercolligeate Competative event on Biotechnology, MP Biotechnology Council.
Second Prize ( Essay Writing Competition)
Hobbies:
Listening music, Painting and photography.
Strength:
Determination, Perseverance, Optimistic, High potential, Quick learning ability
Personal Information:
Name: Palak Shrivastava
Gender: Female
Date of Birth: 06/02/1993
Marital Status: Single
Nationality: Indian
Father’s Name: Late. Sri Ajay Shrivastava
Linguist Ability: English, Hindi
Present Address: Ahsan Building, 1 Hamidia Road, Bhopal (M.P)
Declaration :
I hereby declare that all the details furnished above are true to the best of my knowledge and belief.
PALAK SHRIVASTAVA
References:
Dr. (Ms) Monica Sachdev ( M.Sc Dissertation Supervisor)
Senior Scientist
Endocrinology
CSIR-Central Drug Research Institute( CDRI)
BS-10/1, Sector 10, Jankipuram extension, Sitapur Road,
Lucknow 226031, India
Email id -
Prof. S ubodh Kumar Jain (HOD,Dept. of Biotechnology)
Molecular Biology Lab
Dept.of Biotechnology
Dr.Hari Singh Gour Vishwavidyalaya Sagar -47000, Madhya Pradesh
Email:s-Phone:-
Dr. C.P. Upadhyaya
Plant Molecular Biology, Stress Physiology and Nutrigenomics
Dept. of Biotechnology
Dr.Hari Singh Gour Vishwavidyalaya Sagar - 47000, Madhya Pradesh Email-
Phone:-
Dr. Rajneesh Anupam
Cancer Biology, Viral host –protein interaction Dept. of Biotechnology
Dr.Hari Singh Gour Vishwavidyalaya Sagar -47000, Madhya Pradesh
Email- Phone:-
