Joyce Bernardi

Bernardi Joyce, PhD. Born: 01/08/1987 Education:- Postdoc researcher at the Cardiology department of the Faculty of Health, Medicine and Life Sciences, Maastricht University. Postdoc fellowship on “Arrhythmogenic mechanisms in human channelopaties", University of Milano-Bicocca. Laboratory of Cardiac Cellular Physiology. Ph.D. Program in Translational and Molecular Medicine (DIMET) - XXIX cycle, University of Milano-Bicocca. Laboratory of Cardiac Cellular Physiology. Title of the thesis: “Arrhythmogenic mechanisms in genetic channelopathies”. Master Degree in Medical Biotechnologies, University of Brescia (110/110L) Laboratory of Histology and Pathology. Title of the thesis: “Role of angiogenesis in tumor growth”. Bachelor’s Degree in Biotechnologies, University of Brescia. Laboratory of Pathology. Expertise: Electrophysiology: measurements on freshly isolated and stem cell-derived cardiomyocytes, as well as cellular models (i.e. CHO/HEK). Techniques: Patch-Clamp, Action Potential-Clamp, Dynamic-Clamp, fluorimetric analysis of intracellular calcium. Molecular biology: nucleic acids extraction, DNA and RNA analysis, retrotranscription, PCR and Real Time PCR, proteins analysis. Cellular Biology: primary and immortalized cell cultures, bacterial cultures. Biochemistry: enzymatic assays, Western Blot. Histology: inclusion of biological samples, preparation of histological slices (cryostat and microtome), immunohistochemical analysis and main staining techniques. Publications: 1. Bernardi J, Ronchi C, Mura M, Stefanello M, Badone B, Ronchetti M, Crotti L, Brink PA, Schwartz PJ, Gnecchi M, Zaza A. NOS1AP polymorphisms reduce NOS1 activity and interact with prolonged repolarization in arrhythmogenesis. Cardiovasc Res. 2020 2. Ronchi C, Badone B, Bernardi J, Zaza A. Action potential prolongation, β-adrenergic stimulation and angiotensin II as co-factors in sarcoplasmic reticulum instability. Front Physiol. 2019; 9;9:1893. 3. Ronchi C, Torre E, Rizzetto R, Bernardi J, Rocchetti M. Zaza A. Late sodium current and intracellular ionic homeostasis in acute ischemia. Basic Res Cardiol. 2017;112(2):12. 4. Altomare C, Bartolucci C, Sala L, Bernardi J, Mostacciuolo G, Rocchetti M, Severi S, Zaza A. IKr impact on repolarization and its variability assessed by Dynamic Clamp. Circ Arrhythm Electrophysiol. 2015;8(5):1265-75. Participation at meeting, conferences, courses:         The 39th meeting of ESC working group on cardiac and cellular electrophysiology (EWGCCE) during the EHRA EUROPACE - CARDIOSTIM Congress in Milan, 20-22 June 2015; The 10th annual Meeting of Young Researchers in Physiology (YRP), organized by the Italian Physiology Society (SIF) in Magnano in Riviera (UD), Italy, 5-7 May 2016 (oral presentation); The 5th Lugano Stem Cell Meeting in Lugano, Switzerland, 20-21 June 2016. The 40th meeting of ESC working group on cardiac and cellular electrophysiology (EWGCCE) in Glasgow, United Kingdom, 2-4 September 2016 (poster presentation. Travel grant awarded); The 41th meeting of ESC working group on cardiac and cellular electrophysiology (EWGCCE) during the EHRA EUROPACE - CARDIOSTIM Congress in Vienna, Austria, 17-19 June 2017 (poster presentation). The 5th Italian experience in biomedical research: young minds at work meeting in Desenzano del Garda (BS), Italy, 20-21 October 2017 (oral presentation). The 2nd Translational Cardiovascular Research Meeting in Utrecht, Netherlands, 28-29 June 2018 (oral presentation). PLN Research day in Amsterdam, Netherlands, 2 November 2018.