Jeremhea Santiago

Health Research and Development Information Network Herdin Record #: PCHRD- A new species of greeniopsis merr. (rubiaceae) inferred from morphology and its (nr DNA) sequence data. Researchers Name 1 Daphne Gelera 2 Carl Conrad Catbagan 3 Jeremhea Saliendres 4 Grecebio J. D. Alejandro Role Author Author Author Author Publication Information 1. Publication Type : Publication Sub Type : Title: Date: Page(s): Publisher: Book of Abstracts Book of Abstracts UST College of Science Journal- University of Santo Tomas Abstract A herbarium specimen of Greeniopsis coriacea, a species of the endemic Rubiaceae genus Greeniopsis, is found at the Philippine National Herbarium. However, its identity is anomalous based on the ICBN code due to lack of accompanying morphological descriptions, full illustration, designation of type specimen. Likewise, its phylogenetic relationship with other Greeniopsis species is uncertain. In this morphological and molecular study, genomic DN-A of Greeniopsis coriacea was extracted, amplified, purified, sequenced and analyzed. Thorough morphological examinations, focusing on both vegetative and reproductive parts were also conducted. Morphological examinations based from field observation and herbarium specimens and parsimony analysis of the Internal Transcribed Spacer (ITS) region of the nuclear ribosomal DNA were utilized to 1) provide a full illustration and detailed morphological description of G. coriaceae focusing both on the reproductive and vegetative parts, 2) identify distinct morphological features that delimit Greeniopsis coriacea from the rest of the species, 3) surface phylogenetic relationship between G. coriacea and other species, and 4) assign a type specimen for the species. In accordance to the rules and recommendations imbibed in the ICBN, particularly regarding typification and publication effectivity, the present study changes the invalid name Greeniopsis coriacea and recognizes Greeniopsis wrightii, as a new distinct species. The species is unique by having elliptic leaves in contrast to the common obovate blades, 7-9 pairs only of lateral veins, glabrous inner surface of stipules, shorter inflorescences however bigger flowers (8-11 mm long, 5-7 mm wide ) and fruits ( 6-10 mm long, 4-6 mm wide ) and longer pedicels comparable to that of Greeniopsis megalantha, longer styles and the outer surface of bracts are distinctly puberulous at the base only. Moreover, the topology yielded by the ITS analysis (BS=84%) is concordant with that of morphology wherein G. coriacea and G. megalantha were shown to be somewhat closely related to each other. Powered by TCPDF (www.tcpdf.org)