David Ribas

ACADEMIC BACKGROUND: ▪ 2018 - University of Minho/University of Athens PhD in Molecular Biology ▪ 2013 - University of Minho, School of Sciences Masters in Molecular Genetics (Classification 18 out of 20) ▪ 2011 - Polytechnic Institute of Viana do Castelo Degree in Biotechnology (Classification 17 out of 20) David Ribas 7 th October 1989 PhD Molecular Biology ▪ 2007 - Escola Secundária de Paredes de Coura Basic school (16 out of 20) ACADEMIC AND PROFESSIONAL EXPERIENCE: ▪ 2018 – BosterBio, California, USA FREELANCER Writing of Molecular Biology ebook (https://www.bosterbio.com/wb -ihc-elisa-troubleshootinghandbooks) and product consultant Email:-Driver license: A1, B1, B Skills: • Scientific writing • Microbiology • Bioinformatic analysis • Biostatistics analysis • Molecular docking • Molecular biology • Genetics Professional & Academic Collaborations: ▪ 2018 – Unity Biotechnology, California, USA FREELANCER Biostatistics analysis ▪ 2018 – Memorial Sloan Kettering Cancer Center, New York USA FREELANCER Literature review and scientific planning ▪ 2018 - University of Minho, CBMA RESEARCH FELLOW Structural and functional studies of microbial membrane proteins ▪ 2013 (ongoing) – Red Cross Volunteer ▪- - University of Minho, CBMA PhD student dissertation Structural and functional studies of microbial membrane proteins ▪ 2012/2013 - University of Minho, CBMA Masters student dissertation Studies of carboxylate transporters in yeast ▪ 2011 - INE – Statistics National Institute Consultant ▪ 2010 – University of Minho, CEB Degree student internship Studies of protozoa grazing of bacteria cells 1 7/5/2018 PROFESSIONAL FORMATION: ▪ English formation - BULATS (English certificate) – Wall street English – 2017 ▪ Formation in “management of the cintilation counter -TRI-CARB 4810 TR” – ILC - 2017 ▪ Formation in publication of scientific articles– Elsevier – 2016 ▪ Formation in basic life support and automated external defibrillation – Red Cross – 2014 ▪ Formation in first aid urgent care teams – Red Cross – 2014 ▪ Formation in innovation purification systems of water for laboratorial use – MERCK, s.a – 2013 ▪ Formation in technics of pipetting systems handling – Gilson - 2013 ▪ Formation in “ Formação Pedagógica de Formadores” - 4EMES - 2011 ▪ Formation in Sludge Biotic index – BIOTEMPO - 2010 ▪ Workshop of Agrobacterium tumefaciens transformation – Instituto Politécnico de Viana do Castelo, ESAPL – 2008 PATENTS ▪ Provisional patent application - Reference code: n.º 110312 SCIENTIFIC ARTICLES: ▪ Azevedo-Silva, J., Ribas, D., Castagnoli, D., Sá-Pessoa, J., Athayde, D., Soares-Silva, I., Archer, M., and Casal, M. (2018). P-242 - Exploring the function and structure of the acetate transporter family. Free Radical Biology and Medicine 120, S118. ▪ Ribas, D., Sá-Pessoa J, Soares-Silva I, Paiva S, Nygård Y, Ruohonen L, Penttilä M, and M., C. (2017). Yeast as a tool to express sugar acid transporters with biotechnological interest. FEMS yeast research 17. ▪ Casal, M., Queirós, O., Talaia, G., Ribas, D., and Paiva, S. (2016). Carboxylic Acids Plasma Membrane Transporters in Saccharomyces cerevisiae. Advances in experimental medicine and biology 892, 229-251. ▪ Soares-Silva, I., Ribas, D., Foskolou, I.P., Barata, B., Bessa, D., Paiva, S., Queirós, O., and Casal, M. (2015). The Debaryomyces hansenii carboxylate transporters Jen1 homologues are functional in Saccharomyces cerevisiae. FEMS yeast research 15. ▪ Sá-Pessoa, J., Paiva, S., Ribas, D., Silva, I.J., Viegas, S.C., Arraiano, C.M., and Casal, M. (2013). SATP (YaaH), a succinate-acetate transporter protein in Escherichia coli. The Biochemical journal 454, 585-595. ▪ Niedźwiecka, K., Ribas, D., Casal, M., Ułaszewski, S. (2018). The Cryptococcus neoformans monocarboxylate transporter Cn04 is responsible for increased 3-bromopyruvate sensitivity. Submitted in Applied Microbiology and Biotechnology ▪ Ribas D., Soares-Silva, I., Vieira, D., Sousa-Silva, Sá-Pessoa, J., M., Azevedo-Silva, J., Paiva, S., Viegas, S., Arraiano, S., Paiva, S, Soares, P., Casal, M., (2018). The Acetate Uptake Transporter family motif “NPAPLGL(M/S)” is essential for substrate uptake and binding. Submitted in Fungal Genetics and Biology ▪ Talaia, G., Ribas, D., Casal, M., Diallinas, G., Paiva, S. (2018). Τhe cytosolic domains of Jen1 are critical for subcellular localization, function and turnover. Submitted in Molecular Microbiology ▪ Ribas, D., Soares-Silva, I., Casal, M. (2018). Membrane transporters: the key to boost microbial production of 7/5/2018 organic acids. In preparation. 2 ORAL COMMUNICATIONS: ▪ Ribas, D., Soares-Silva, I., Azevedo-Silva, J., Sá-Pessoa, J., Diallinas, J., Paiva, S., Casal M., Structural and functional characterization of AceTr transporter family members, 33rd International Specialised Symposium on Yeast (ISSY33), Cork 26-29 June 2017 ▪ Ribas, D., Soares-Silva, I., Azevedo-Silva, J., Sá-Pessoa, Paiva, S., Casal M. Uncovering the critical role of “NP(A/V)P(L/F)GL(M/S)” AceTr family motif in acetate uptake, XLI Jornadas Portuguesas de Genética, 8-9 June, Aveiro, Portugal, 2017 ▪ Ribas, D., Sá-Pessoa, J., Paiva, S., Ruohonen L., Penttilä, M., Casal, M. Yeast as a tool to express carboxylic acid transporters with biotechnological interest. SMYTE 34 – Small Meeting in Yeast Transport and Energetics, 29 August-1 September 2015, Crete, Greece. ▪ Ribas, D., Athayde D., Azevedo-Silva, J., Archer, M., Casal, M. Uncovering the molecular structure of the AceTr transporter family. SMYTE 34 – Small Meeting in Yeast Transport and Energetics, 29 August-1 September 2016 Crete, Greece. ▪ Ribas, D., Athayde D., Archer, M., Casal, M. Uncovering the molecular structure of the AceTr transporter family. XXXVI Jornadas Portuguesas de Genética, 2-3 June 2016, Coimbra. ▪ Ribas, D., Soares-Silva, I., Foskolou, Z., Barata, B., Bessa, D., Paiva, S., Queirós, O., Casal, M. TheDebaryomyces hansenii carboxylate transporters Jen1 homologues are functional in Saccharomyces cerevisiae. 33 rd Small Meeting in Yeast Transport and Energetics 2015, 2124 July, Lisbon, Portugal. ▪ Neto, M., Ribas, D., Lima, N., Mota, M., Nicolau, A. Environmental Microbiology and Biotechnology – Protozoa Grazing evaluation: a novel way to assess wastewater treatment performance? Microbiotec’11 – Portuguese Society for Microbiology, Braga, 2012. ▪ Ribas, D., Neto M., Nicolau, A. Protozoa grazing evaluation: a novel way to assess wastewater treatment performance. 2nd Scientific Meeting of the Institute fo r Biotechnology and Bioengineering, Universidade do Minho, Braga, 2010. 7/5/2018 3 POSTER PRESENTATIONS: ▪ Ribas. D., Soares-Silva, I, Azevedo-Silva J., Silva M., Sá-Pessoa J., Paiva, S., Casal M, Structural and functional characterization of AceTr transporter family, MICROBIOTEC’ 17, 79 December 2017, Universidade Católica Portuguesa, Porto, Portugal. ▪ Azevedo-Silva J., Athayde D., Ribas, D., Sá-Pessoa, J., Archer, M., Casal, M. “Unravelling the structure of Acetate transporters in bacteria: expression and purification studies towards crystallography”. XLI Jornadas Portuguesas de Genética, Aveiro (Portugal). June 8-9, 2017 ▪ Sousa-Silva, M., Ribas D., Casal, M., Soares-Silva, I. Heterologous expression ofCandida utilis carboxylic acid transporter homologs in Saccharomyces cerevisiae, XLI Jornadas Portuguesas de Genética, 8-9 June, Aveiro, Portugal, 2017 ▪ Vieira, D., Soares-Silva, I., Ribas, D., Azevedo-Silva, J., Casal, M., Soares, P. A tool for the extraction of relevant and representative homologues of a given gene: the example of a transmembrane protein from Eukaryotic and Prokaryotic genomes, MICROBIOTEC’ 17, 7-9 December 2017, Universidade Católica Portuguesa, Porto, Portugal. ▪ Ribas, D., Sá-Pessoa, J., Paiva, S., Soares-Silva, I., Nigard, Y., Ruohonen L., Penttilä, M., Casal, M. Yeast as a tool to express carboxylic acid transporters with biotechnological interest. XIX National Congress of biochemistry 8-10 December 2016, Guimarães. ▪ Ribas, D., Athayde D., Sá-Pessoa, J., Azevedo-Silva, J., Viegas, S., Archer, M., Casal, M. Uncovering the molecular structure of the AceTr transporter family. XIX National Congress of biochemistry 8-10 December 2016, Guimarães. ▪ Ribas, D., Sá-Pessoa, J., Paiva, S., Ruohonen L., Penttilä, M., Casal, M. Yeast as a tool to express carboxylic acid transporters with biotechnological interest. PYFF6 - Sixth Conference on Physiology of Yeasts and Filamentous Fungi 11-14 July 2016, Lisbon. ▪ Ribas, D., Athayde D., Archer, M., Casal, M. Uncovering the molecular structure of the AceTr transporter family. PYFF6 - Sixth Conference on Physiology of Yeasts and Filamentous Fungi 11-14 July 2016, Lisbon. ▪ Ribas, D., Sá-Pessoa, J., Paiva, S., Ruohonen L., Penttilä, M., Casal, M. Yeast as a tool to express carboxylic acid transporters with biotechnological interest. XXXVI Jornadas Portuguesas de Genética, University of Coimbra, 2-3 June 2016, Coimbra ▪ Ribas, D., Soares-Silva, I., Foskolou, Z., Barata, B., Bessa, D., Paiva, S., Queirós, O., Casal, M. The Debaryomyces hansenii carboxylate transporters Jen1 homologues are functional in Saccharomyces cerevisiae. MicroBiotec 2015, 10 – 12 December, Évora, Portugal. ▪ Ribas, D., Soares-Silva, I., Foskolou, Z., Barata, B., Bessa, D., Paiva, S., Queirós, O., Casal, M. The Debaryomyces hansenii carboxylate transporters Jen1 homologues are functional in Saccharomyces cerevisiae. III Symposium of the PhD Programme on Molecular and Environmental Biology (PDBMA) 2015, 26 November, Braga, Portugal. ▪ Ribas, D., Soares-Silva, I., Foskolou, Z., Barata, B., Bessa, D., Paiva, S., Queirós, O., Casal, M. Heterologous expression of Debaryomyces hansenii Jen1 homologues in Saccharomyces cerevisiae. XXXIX Jornadas Portuguesas de Genética, 25 – 27 May 2015, Braga, Portugal. ▪ Ribas, D., Casal M. Uncovering the molecular structure of the AceTr transporter family: the role of conserved amino acids residues in acetate uptake. II Symposium of the PhD Programme on Molecular and Environmental Biology, Braga, 2014. ▪ Neto, M., Ribas, D., Lima, N., Mota, M., Nicolau, A. Protozoa grazing evaluation: a novel way to assess wastewater treatment performance? Poster Session: Water and Industry 2011 IWA Specialist Conference Chemical industries, Valladolid, 2011 7/5/2018 4 Degree certificate 7/5/2018 5 Master certificate 7/5/2018 6