Antoine Torchiat

ANTOINE TORCHIAT Tel: -- Route du Jura 51, 1700 Fribourg, Switzerland PROFILE I am a PhD Student In chronobiology deeply interested in biology. I have spent five years of study in biomedicine related field following my research interest. I developed a particular interest in human diseases which increased during an internship in a cancer center in France. Five years of practical experience in the biological and pharmaceutical field gained through internships and university. As a PhD student I know how to manage my time, working hard 7/7. I always wanted to work in a field combining my studies (biology and medicine) and my center of interest (marketing/business) that is why I'm reading a lot of books about psychology and marketing and I'm applying to your position. Strong communication and team working skills developed through internship projects presentation, tutorial discussions and extra- curricular activities. EDUCATION MSc Biomedical & Molecular Sciences | 2020 – 2021 (distinction 2:1) School of Life Sciences, University of Dundee, Dundee, UK Modules studied: - Cell Biology and Disease (Embryogenesis, Functions & structure of cells, Stem cels signaling & communication in a normal or disease context) Molecular Basis of Diseases: Genetics, Epigenetics and Genomics (DNA structure & modifications, genome stability related to diseases such as breast, lungs or cardiovascular cancers & diabetes) Scientific Method and Skills (Scientific knowledge inherent in the research profession, ethics, integrity, data management, Statistics) Advanced Technology Platforms (Computing and bioinformatics, Imaging and manipulation of cells, Analysis of proteins and proteomes) Therapeutics (Basics of Immunology, Development of new and improved treatments for disease, Cell therapies & regenerative medicine, CAR T cells, Drug regulation) - MSc Research Project (Design, execution and communication of a substantive laboratory-based or focused research project on an area of current world- class research in biomedical and molecular sciences.) Professional degree in the pharmaceutical industry | 2018 - 2019 University of Franche Comté, UFR sciences médicales et pharmaceutiques Besançon, France Modules studied: - Products of natural origin and from biotechnology (Place of natural substances in pharmacy and cosmetology, Principles of extraction, Microbial, animal and plant biotechnologies) - Pharmaceutical formulation (Physicochemistry of excipients and ingredients, Administration routes, Bioavailability and formulation) Master in Biology |- Cell & Molecular Signaling University of Franche Comté, UFR Sciences et Techniques, Besançon France Modules studied: - Immunology (non-adaptive immunity, inflammatory reaction, adaptive immunity, soluble proteins of adaptive immunity, Interferons) - Regulation of gene expression in eukaryotes (Chromatian, transcriptional, post-transcriptional, translational and post-translational level) Bachelor of Science | 2015 - 2017 University of Franche Comté, UFR Sciences et Techniques, Besançon France Modules studied: - Biochemistry (Basics of a biological reactions & metabolism pathways) - Physiology (Major physiological functions in humans, Interpret experimental data to consider their modeling) Cellular biology (basic techniques of separation and analysis of biomolecules and their interest) Medical Studies | 2013 – 2015 Modules studied: - Atom - Biomolecules - Genome - Bioenergetics - Metabolism (Biochemistry and molecular biology, Physiology and cell biology, Biophysics and nuclear medicine, Nutrition, Genetics and biochemistry, Biophysics - biomathematics, Chemistry, Physiology) The cell and tissues (Pathological anatomy and cytology, Biochemistry and molecular biology, Cellular biology, Developmental and reproductive, Biology and medicine, medical gynecology, Cytology and histology, Neuroscience) Organization of devices and systems : physical bases of exploration methods (Functional aspects, Biophysics - biomathematics, General and mineral chemistry, physical chemistry, Study methods Evaluation of analytical methods applied to life and health sciences (Biophysics - biomathematics - nuclear medicine, Biostatistics, medical informatics and communication technology, Mathematics and physics) Initiation to knowledge of the drug (Law and health economics, Galenic pharmacy, Pharmacology and pharmacokinetics, Basic pharmacology; clinical pharmacology, Medical reanimation - Therapeutic; emergency medicine) Health, Society Humanity - Human and social sciences (Anthropology, Law and political science - economics, Epidemiology, health economics and prevention, Contemporary history, Psychology, Sociology) RESEARCH EXPERIENCE PhD project: ‘’Per protein phosphorylation and their interactome” at the Université de Fribourg supervised by Pr Urs Albrecht | Feb 2022 We have identified that Cyclin-dependent kinase 5 (CDK5) interacts with PER2 and phosphorylates it at Ser394 to maintain its stability and transport it into the nucleus. PER1 & PER2 genes are involved in regulating distinct and opposite biological functions. Most of PER proteins phosphorylation sites are not characterized, especially for PER1. We aim first to find the potential CDK5 dependent phosphorylation site in PER1 & investigate the interactome of PER1 depending on CDK5 phosphorylation site. Our preliminary data indicate that PER1 and CDK5 can interact in vitro in cells. We plan to do invitro kinase essay followed by mass spectrometry to identify PER1 amino acid(s) phosphorylated by CDK5. The phosphorylation site(s) will be mutagenized followed by a kinase essay. We will produce an appropriate antibody to test CDK5-dependant PER1 phosphorylation, address the molecular meaning of the identified CDK5-dependant phosphorylation site of PER1, the protein stability, its half-life and its period length. We will also see its impact on protein oscillations of clock genes such as CLOCK, BMAL1, PER2, CRY and potential changes in the transcriptional activity. Finally, we will characterize CDK5 dependent phosphorylation site of PER1 in term of temporality and spatial nature in the suprachiasmatic nuclei (SCN) in vivo in mice. MSc Biomedical & Molecular Sciences Research project: ‘’Validation and characterization of one of the potential substrates of Mpk1 kinase” at the Medical Research Council Protein Phosphorylation and Ubiquitylation Unit supervised by Dr Adrien Rousseau | May - August 2021 I used well-established methods for the analysis of MPK1 signalling, including methods to detect MPK1 activation in cells using phosphorylation-specific antibodies raised against a motif known to be present only in activated MPKs, and methods to monitor if a given protein is being phosphorylated in cells such as PhosTag gels. The key goals of this project are: To estimate the activation level of Mpk1 kinase. To validate that potential Mpk1 substrates identified are less phosphorylated in cells lacking Mpk1. To identify the sites of Mpk1 phosphorylation on validated substrates. To investigate the potential role of substrate phosphorylation in stress responses. To use Crispr/Cas9 to modify these motifs and conclued on their roles. Professional degree in the pharmaceutical industry; internship | March – July 2019 Jean-François Leclerc Center, Dijon, France Supervisors: Dr. Isabelle Desmoulins Clinical research assistant in a clinical trial project (MONARCH3) focussed on a new drug target in breast cancer. This internship allowed me to apply and expand on my knowledge and skills in immunology, genetics, physiology and data analysis, and additionally to learn to communicate effectively though my interaction with patients. Master internship | January - March 2018 Bacteriology Laboratory UMR 6249 Besançon, France Supervisors: Dr. Katy Jeannot and Pr. Patrick Plésiat Project: Functional study of new alkaloids produced by Pseudomonas aeruginosa In this project, I used overlap-extension PCR and required the help of the Paris National Museum of Natural History to investigate which gene was responsible for the secretion and modeled in 3D this alkaloid. - Research skills include qPCR, PCR, antibiotic resistance testing, bacterial cultures, cloning, genes knockout/deletion, DNA/RNA extraction / quantification Skills -Languages: Fluent in French (native) and English, basic communication skills in Spanish -Software: Experienced in data analysis software such as R Studio, usage of Microsoft OfficeSuite.SkillsinAdobeAftereffect&Photoshop. -Science related: Cell Culture, Data Analysis, PCR, Crispr/Cas9 INTERESTS AND ACTIVITIES -Football: former national player, international tournaments -) -Reading: Novels (The Truth : Neil Strauss, 1984 : George Orwell…). Scientific reading: subscription for Science magazine REFERENCES Dr. Joost Zomerdijk, Reader (Associate Professor) in Gene Regulation and Expression, Programme Lead of MSc Biomedical & Molecular Sciences courses, School of Life Sciences, University of Dundee, Dundee, UK. Email:-Tel : +44 -, int ext 84242 Pr. Jenny Woof, Professor and Associate Dean (Quality & Academic Standards), School of Life Sciences, University of Dundee, Dundee, UK. Email:-Tel : +44 -, int ext 83389 Pr. Tom Owen Hugues, Professor of Chromatin Structure and Function, MSc Biomedical & Molecular Sciences, University of Dundee, Dundee, UK. Email:- ­­