Allyssa Walburn

Allyssa Walburn Summerville, SC 29486-- Authorized to work in the US for any employer Work Experience Quality Control Charles River Laboratories-Charleston, SC October 2022 to Present Description: Quality Control Analysts from Charles Rivers develop and implement testing/inspection procedures. Raw material, chemicals, and/or finished product are tested to quality controls to ensure compliance with quality standards and applicable government regulations. Analyst audit testing results to determine if product specifications are met. Environmental monitoring of LAL (Limulus Amebocyte Lysate) and Crude Lysate production areas to ensure appropriate conditions are maintained. They advise operating units on quality control standards for raw materials, chemicals, packaging materials, and finished products. Recommends corrective action where necessary and develops plant quality control manuals. Responsibilities: Collect and log samples of Limulus Amebocyte Lysate (LAL) into the lab. Create serial dilutions of U.S. Reference Standard Endotoxin, used to perform a various of quality control testing on samples from batches (potency, moisture, pH, and sterility). Perform gel-clot tests on samples from batches to test potency accuracy, at varies time points and conditions. Sample manufacturing personnel and surfaces in clean rooms to ensure proper clean room practices are followed. Take viable and non-viable air samples in cleans rooms, to ensure minimum air particles/contaminants are present. Skills: cGMP, SOPs, Growth Promotion Testing, Gel-clot Testing, Viable and Non-viable air collecting. Research Associate Luceome Biotechnologies-Tucson, AZ July 2020 to August 2020 Tucson, AZ- July 2020 to August 2020 Description: Luceome focuses on using cell-free split-protein assays on kinases, utilizing luciferase fragment complementation. The luminescence detection from these assays are sensitive, with low background interference, allowing identification of target and off-target effects of drug candidates. Responsibilities: Research Associate, working a minimum of 40 hours a week in this lab. Used biochemical techniques to grew and harvested Circular DNA plasmids for different target kinases. Performed Polymerase Chain Reaction (PCR) experiments to create linear DNA, then used PCR experiments to create RNA from those products. Performed translation experiments to get kinase proteins for the split-protein assay. Performed split-protein assays for targeted kinases, using different inhibitors. Skills: PCR, RNA Translation, Split-protein Systems, Microsoft Excel, Electrophoresis Gels, DNA Purification, RNA Purification, Biochemistry Assays Volunteer Student Researcher Dr. Henk Granzier Physiology Lab-Tucson, AZ January 2019 to May 2020 Description: Dr. Granzier focuses on elucidating the structure and function of titin and nebulin, two large filamentous proteins found in muscle. The lab uses a range of model systems with a major focus on knock-out and transgenic mouse models. The techniques that are used ranged from single molecule mechanics, (immuno) electron microscopy, exon microarray analysis, in vitro motility assays, low angle X-ray diffraction, cell physiology (including calcium imaging), muscle mechanics, and isolated heart physiology. Responsibilities: Volunteered a minimum of 20 hours a week in this lab. Lead a project that studied an isoform of titin in knock-out and wild-type mice models. Maintained mice housing, performed mice dissections, gel analysis, and computer analysis using various of programs (GraphPad Prism, MATLAB, Microsoft Excel, and more). Skills: Mice dissections, Electrophoresis Gels, Microsoft Excel, PowerPoint, Word, GraphPad Prism, MATLAB, OneDScan, Student Researcher Dr. Michael Marty Biochemistry and Analytical Chemistry Lab January 2018 to October 2018 Description: Dr. Marty is using mass spectrometry to examine lipoproteins using nanodisc complex. Nanodisc mimics the properties of a membrane in our cells, so it is ideal for examining the correct complex of membrane proteins in our body. Responsibilities: Shadowed a Post Doc, Dr. Rohrbough, in Dr. Michael Marty's lab. Performed sample preparations for protein calibration tests on varies equipment (Size Exclusion and Nickel column-FPLC system), created needles for the mass spectrometer, ran samples of prepared nanodiscs, and observed how to interpret data from a mass spectrometer. Used biochemical techniques to grow, harvest, and purify proteins using for numerous projects. Skills: Operated an Autoclave, a Microfluidizer, a Centrifuge, a Mass Spectrometer, a Size Exclusion column, and a Nickel column- FPLC system. Utilized Microsoft Excel, PowerPoint, and Word. Tutor/Mentor University of Arizona ASEMS-Trio-Tucson, AZ February 2018 to May 2018 Responsibilities: Tutored College Level Math, Biology and Chemistry. Objective was to give students secondary help in their classes as well as provide them with different techniques, skills, and resources for them to succeed. Skills: I demonstrate problem-solving skills, the ability to mentor students, and the ability to maintain a friendly healthy environment for my students. Tutor/Mentor Pima Community College-Tucson, AZ August 2016 to March 2017 Responsibilities Tutored College Level Math and Chemistry. Objective was to give students secondary help in their classes as well as provide them with different techniques, skills, and resources for them to succeed. Accomplishments: I earn my level one certification in Mentoring/Tutoring. Skills: I demonstrate problem-solving skills, the ability to mentor students, and the ability to maintain a friendly healthy environment for my students. Education Master's degree in Biotechnology Johns Hopkins University - Baltimore, MD January 2024 to Present Bachelor's in Molecular and Cellular Biology University of Arizona - Tucson, AZ August 2018 to May 2020 Bachelor's in Biochemistry University of Arizona - Tucson, AZ August 2017 to May 2020 Associate's degree in Science and Liberal Arts Pima Community College - Tucson, AZ August 2015 to May 2017 Skills • PCR • RNA Translation • Microsoft Office • Assays • Laboratory Experience • MATLAB • Spectroscopy • Protein purification • Chromatography • Molecular biology • Retail sales • Customer service • Research & Development • Calibration • High-Performance Liquid Chromatography • GLP • CGMP • Research • Assays • Laboratory experience • PCR • Quality control • Mentoring • Customer service • Tutoring • Microsoft Excel • Microsoft Outlook • Microsoft Office • Manufacturing