Afifa Butt

AFIFA BUTT --Education University of The Punjab, School of Biological Sciences M Phil (Molecular Biology) CGPA: 3.7 Lahore, Pakistan University of The Punjab, Microbiology & Molecular Genetics Bachelor’s in microbiology and Molecular Genetics CGPA: 3.69 Lahore, Pakistan - - Academic Experience Research Project Lahore, Pakistan Final year project in Bachelors (university of Punjab, Lahore.) Thesis Title: Diagnostic discrepancies among MRSA isolates in pediatrics. Community Project – Assessment of Awareness of Dementia Among the Population of Punjab. (Publication in process) – Group of 3 members worked together to make report. Poster Presentation Presented research poster at conference of microbiology and molecular genetics MMG2018 at the University of Punjab. Technical Proficiency in Lab Microbiology – Media preparation for Bacterial Cultures, Serial Dilution Method for Bacterial Cultures, Pouring, Plugging, Slant preparation & Streaking on plates, Sub Culturing of Microbes. – Isolation and Culturing of microbes from Soil Sample (Through Serial Dilution Method), from Water Sample & from Air. (Through Exposure Method). – Biochemical Test for Screening of Microbes. – Isolation of antibiotic resistance microbe from soil. – Determination of cell size of microorganisms. Staining (endospore, capsular and gram staining for bacteria), Kinetics of Microbes, Antibiotics Sensitivity Test. – Inoculation and culturing of E. coli, Revival of culture, Microbial assay of antibiotics by diffusion method. Molecular Biology – Isolation of DNA from microbes, to check purity of DNA, Plasmid isolation from microbes, Agarose Gel electrophoresis, Amplification of Genomic DNA by PCR (polymerase chain reaction), SDS, paper chromatography. Food & Industrial Microbiology – Estimation of protein by biuret method, Food hygiene testing, Detection of adulteration in different food items, MBR test (methylene blue reduction), Amylase production, Wine production from fruits juice, Sauerkraut production, Bacteriological testing for water. Additional – – – Microsoft Word, Power point Interests: Painting, Creativity, Management Language: Urdu, English