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NEIL S. MONDUGAR
LAGUNA STATE POLYTECHNIC UNIVERSITY
SINILOAN, LAGUNA
INTRODUCTION
• HEALTH
• Refined sugar’s harmful effects on the human body
• Stevia helps to lower blood sugar level
• Philippines is included in the top 15 countries worldwide in
diabetes prevalence
• STATUS
• Only a handful of studies, BAR with Dr. Quimio and UPLB
with Dr. Paningbatan
• Only few nurseries in the country grow the herb
• ALTERNATIVE SOURCE
• Sweeter than sugar
• One hectare stevia is equal to 36 hectares of sugar cane
• Healthier than sugar and other artificial sweeteners
CONCEPTUAL FRAMEWORK OF THE STUDY
I n d e p e n d e n t
I.
V a r i a b l e s
Main Plot Factor - Ethanol
source
Dependent Variables
Parameters to measure:
a. Degree brix
b. Specific gravity
II.
a. Vodka
c. pH
b. Lambanog from coconut
d. Alcohol content
c. Lambanog from sasa
e. Yield (volume)
Sub-Plot Factor – Extraction
time
a. 24 hours of extraction
b. 30 hours of extraction
c. 36 hours of extraction
RESEARCH DESIGN
3 X 3 SPLIT PLOT DESIGN IN RCBD
4 REPLICATIONS
36 TOTAL SAMPLES AS
EXPERIMENTAL UNITS
RESEARCH PROCEDURE
PLANT PROPAGATION
HARVESTING OF LEAVES
AIR DRYING OF LEAVES
STRIPPING OF LEAVES FROM STEM
WEIGHING OF LEAVES
CHOPPING OF LEAVES
POURING OF SOLVENT
STORING FOR THE ASSIGNED TIME
FILTERING AND MEASURING OF VOLUME
DATA GATHERING (BRIX, PH, ALCOHOL CONTENT, SPECIFIC
GRAVITY)
DATA ANALYSIS USING SAS SOFTWARE
PURIFICATION
COOLING AND STORING
Lambanog from coconut
Lambanog from sasa
Gilbey’s vodka
RESULTS AND DISCUSSIONS
Table 1. Interaction and Main Effects of Source and Time on pH of the Extract
Ethanol
Source
A. 4.5
B. 3.6
C. 6.5
Lambanog
from coconut
Lambanog
from nipa
(sasa)
Gilbey’s
Vodka
Mean
__
24 Hours
TIME_____________
30 Hours 36 Hours
Mean
5.850
5.375
5.250
5.492b
5.300
4.950
4.750
5.000c
6.525
5.625
5.950
6.033a
5.892a
5.317b
5.317b
Source
F Value
Source
Time
Interaction
Coef. Var. = 4.91
-
P Value
< 0.0001