Boniface

Artemisia annua Variety comparison Table of contents Introduction .............................................................................................................................. 2 I. Materials ............................................................................................................................ 2 I. 1. nursery ..................................................................................................................................... 2 I. 1. 1. Experimental sites ................................................................................................................ 2 I. 1. 2. Nursery technical itinerary .................................................................................................. 2 I. 1. 3. Agricultural inputs ............................................................................................................... 2 I. 1. 4. Agricultural equipment ........................................................................................................ 3 I. 2. transplanting ............................................................................................................................ 3 I. 2. 1. Experimental device ............................................................................................................. 3 I. 2. 2. Technical itinerary ............................................................................................................... 4 I. 2. 3. Agricultural inputs ............................................................................................................... 5 I. 2. 4. Agricultural equipment ........................................................................................................ 5 I. 3. II. II. 1. Observation sheets ................................................................................................................... 5 Methods .......................................................................................................................... 6 Parameters studied ................................................................................................................... 6 III. human resources ............................................................................................................ 7 IV. Implementation period ................................................................................................. 7 V. Construction budget ...................................................................................................... 7 1 Introduction Bionexx begins to decrease the number of varieties use by identifying the one that is best for eachZone. Last year, experimentation on varietal comparison did not do well because of installation delays. For this year, we decided to re-experiment by selecting the two major producing sectors of each Zone. The experimentation will be installed on the terroirs mainly exploited by these sectors and also corresponding to the times of the peak transplant. The aim is to have the best possible condition corresponding to the sector's production. Indeed, this experiment aims to compare varieties by considering the abiotic conditions of Artemisia annua (climate and soil physicochemical property). I. Materials I. 1. nursery I. 1. 1. Experimental sites Four varieties will be planted on a farmer's nursery in each area. Two boards per variety will be to prepare eight boards per Zones. The varieties to compare are: B07, B16 or B63 (EBA F2), B25, B30. The selection criteria for farmers are: • Serious and motivated nursery to follow instructions and advice on experimentation. • Flood-free and fertile plot during nursery cycle (75 days) • Area of 60m2 by Zones • Motorbikes accessible or car at no more than five minutes’ walk or 350m. • The owner is not a Peasants Relay (PR) I. 1. 2. Nursery technical itinerary The technical nursery route follows the techniques in the Artemisia Guide sheet: DP-016. This fact sheet explains the steps to follow from the preparation of the soil to the snatch. I. 1. 3. Agricultural inputs Organic manure and shade materials will be the responsibility of farmer nursery suppliers, mineral fertilizers (NPK) and insecticide will be the responsibility of Bionexx. Seeds will be sent to the areas by the Farmers Technical Optimization Manager. 2 I. 1. 4. Agricultural equipment Agricultural equipment for the installation and treatment of nurseries will be the responsibility of the nursery providers: angady, sobika, sprayers ... I. 2. Transplanting I. 2. 1. Experimental device Seedlings from the nursery only are to be transplanted. Plots to be installed are explained by the following Table 1 Table 1 Distribution of transplant For each site, the experimental device is of the randomized type.1 Plots are separated from a 1 m driveway. Each elementary plot is on the order of one are. Table 1 Distribution of transplant plots Zone Pa PF PV PB Pu sector soil Nb TT Variety - paddy field Baiboho paddy field paddy field paddy field paddy field Baiboho paddy field Baiboho Baiboho - - Elemental surface (a- Repetition Total (a) - - Figure 1 Example of parcel layout 1 Putting plots on a random block 3 The chosen farmer must have at least 9 are of land. The criteria for choosing plots and peasants are: • Serious and motivated peasants to follow instructions and advice on experimentation. • Flood free plot during the culture cycle • Area at least nine are for one farmer. • Motorbikes accessible or car at no more than five minutes’ walk or 350m. • The owner is not a Peasants Relay (PR) Transplants will begin as soon as the seedlings on the nurseries are ready. I. 2. 2. Technical itinerary The technical route follows the technical sheet DF-074 and DP-129 which explains to the farmers the preparation of the soil until the harvest of theArtemisia annua. However, few points are added to this experimentation: After plowing and harrowing, setting up the holes with the 60-60cm spread requires attention. ✓ Draw the planting lines with a spread radius 60cm or put a long rope stretched with knots 60cm apart. ✓ With the radiator, you just have to cross the lines perpendicularly and make holes on each intersection of the lines. ✓ With the rope, you have to stretch the rope and make holes corresponding to each knot, then move 60cm later to have a new line. ✓ Leave a 1m driveway after completing 270 holes to form a small plot and so on. ✓ Put in each hole two handfuls of fertilizers (or 0.5 kg of dry fertilizers) mixed with the soil according to the defined device (one is per treatment). ✓ At the transplant, you have to make sure the holes are full. Table 2 General Interviews Interviews Plant health treatment - Insecticide - Insecticide - fungicide watering Weeding/ hoeing period Dose transplanting 2,5 cc/are During the cycle in case of 2,5 cc/are remarkable attacks During the cycle in case of 60g/are remarkable attacks 1st week 15-18feet/bucket 4th 2 to week 12-15feet/bucket 5th to 8th week 10ft/bucket 12th 9 to week 5 feet/bucket Every 30 days frequency 1 time 1 time 1 time Every day 3 times a week 2 times a week 1 time/week 1 time 4 ✓ Harvest period:150 days after transplanting. I. 2. 3. Agricultural inputs The following table shows the agricultural inputs to be prepared: quantity Observation Organic smoke 21.6t 4.32t per Zone NPK (kpk) 320kpk 64kpk per Zone Urea(kpk) 320kpk 64kpk per Zone 400cc 80cc per Zone Insecticide (2.5cc/are) Plantstones (nb) 96 000 1,200 seedlings per variety per Zone I. 2. 4. Agricultural equipment I. 2. 4. 1. bucket The bucket is used for watering. A bucket of 10l per two are plot is given to the farmer for watering, i.e. 80 buckets in total. I. 2. 4. 2. decameter A decameter is used to check the spread of the planting and the hole sizes. I. 3. Observation sheets Observation sheets and/or smartphones will also be used to collect data. Notebook for recording activities carried out by owner farmers. 5 II. Methods II. 1. Parameters The following table illustrates the tasks and factors to be studied for this experiment, the periods, and frequencies to have the necessary data. stadium nursery Spots Germinative energy (visual germination rate) Counting seedlings Maturity time Measuring height transplanti ng period − S+7 − S+15 frequency 1 time S-20 to S-25 S+35 S+45 lifting S + 45 lifting Every 30 days 1 time 1 time Phenological measurement (height, diameter, number of branches) Mortality rate − R+15 to R+20 − R+30 to R+35 − R+105 to R+110 Observe the proportion of seedlings capable of transplanting by planks 1 time Choose 10 plants on each diagonal of the plot 1 time − Mark by pricking the empty holes before replacing seedlings up to R-20. − No seedling replacements are allowed after R-20. − Only seedlings coming from nursery varietal comparison will be used for replacement and in agreement with the ROTP team. Sensitivity to disease − − − − Drought sensitivity − Every visit 1 time Fresh weight MS Weight At harvest purchase 1 time 1 time Artemisinin rate Remarks Observe the proportion of seedlings sprouted by planks 1 time R+105 harvest purchase 1 time Systematic observation every 30 days − Mark by stage for rusts − 0 and 1 for other diseases with attack percentage on the plot − Observe the general state of culture The purchase will be separately from the Zones programs. Every time you visit, you must take pictures of the plot. 6 III. Human resources ✓ The peasant manager validates the test protocol and the related budget, ✓ The farmer's technical optimization manager establishes the test protocol and ensures its implementation with the peasant team until the results obtained are processed. It ensures the general monitoring of activities related to this experimentation, ✓ The peasants who own the field provides the hands of works for the setting up of plots, general maintenance, harvesting and post-harvest processing, recording in his notebook all activities carried out with dates and the number of hands of work. It guarantees compliance with the technical record and the protocol. IV. Implementation Period The nursery implementation periods are aimed at peak transplanting each sector. Indeed, the table below shows the planting schedule for each Zone: S10 S13 S15 S17 S20 Pa PF PV PB Pu Pr Pr Pr Pr Pr S21 S23 S25 S28 S29 S41 S44 S46 S49 S50 S52 Pep Rep Rec Rap Pep Rep Rec Rap Pep Rep Rec Rap Pep Rep Rec Rap Pep Rep Rec Rap Legends: − Pr: Preparation and validation protocol − Pep: Setting up nursery − Rep: Setting up transplanting − Rec: Harvest and packaging − Rap: Report V. Construction budget − The hands of works are entirely at the expense of the peasants. − The products obtained are all attributed to the owners. − Ordinary agricultural materials such as angady, sobika, harrow or plow are also entrusted to farmers. 7 The following charges are on behalf of Bionexx/Research and Development: designation unit quantity PU (Ar) Amount (Ar) Nurseryman performance Boards 40 15,000 600,000 Organic manure Bags 432 16,389 7,079,842 (transplanting) Manure transport Bags 432 2,000 864,000 Participation rent parcels Tt 20 15,000 300,000 Plot panel in the 200 9,000 1,800,000 Total to buy 10,643,842 seed Bags 40 2,500 100,000 bucket room 80 2,250 180,000 Insecticide cc-,500 NPK Kpk-,600 urea Kpk-,000 Total sub at Bionexx 666,100 Total expenses 11,309,942 Observation To buy At Bionexx 8